From fff06bf77d4db8acfc9bb1dad49500238b6df0c4 Mon Sep 17 00:00:00 2001 From: Charles Plessy Date: Mon, 19 Mar 2018 17:02:05 +0900 Subject: [PATCH] Biblio. --- biblio/28327113.mdwn | 10 ++++++++++ tags/template_switching.mdwn | 4 ++++ 2 files changed, 14 insertions(+) create mode 100644 biblio/28327113.mdwn diff --git a/biblio/28327113.mdwn b/biblio/28327113.mdwn new file mode 100644 index 00000000..a3dfc9d5 --- /dev/null +++ b/biblio/28327113.mdwn @@ -0,0 +1,10 @@ +[[!meta title="Low-cell-number, single-tube amplification (STA) of total RNA revealed transcriptome changes from pluripotency to endothelium."]] +[[!tag template_switching reverse_transcription]] + +Lee YH, Hsueh YW, Peng YH, Chang KC, Tsai KJ, Sun HS, Su IJ, Chiang PM. + +BMC Biol. 2017 Mar 21;15(1):22. doi:10.1186/s12915-017-0359-5 + +Low-cell-number, single-tube amplification (STA) of total RNA revealed transcriptome changes from pluripotency to endothelium. + +[[!pmid 28327113 desc="Poly-A tailing followed by template switching. Increased dNTPs to 2 mM and Mg2+ to 9 mM to favour terminal addition of nucleotides. TSO and RT primers are at 1 μM final."]] diff --git a/tags/template_switching.mdwn b/tags/template_switching.mdwn index 1f7635ed..0e6b0d8f 100644 --- a/tags/template_switching.mdwn +++ b/tags/template_switching.mdwn @@ -12,4 +12,8 @@ can extend a linker with the sequence of a small RNA via a template switching reaction. (That is: a sRNA can play the same role as a TS oligonucleotide.) + - [[Lee et al (2017)|biblio/28327113]] increased the efficiency of template + switching non-capped molecules by increasing dNTPs to 2 mM and + Mg2+ to 9 mM. + [[!inline pages="tagged(template_switching)" limit=0]] -- 2.47.3