From f455fe7a8e3983e5dd2a7c4cf4384a81dda31b2d Mon Sep 17 00:00:00 2001
From: Charles Plessy <https://launchpad.net/~plessy>
Date: Mon, 18 Aug 2014 17:32:14 +0900
Subject: [PATCH] =?utf8?q?Hier=20dans=20le=20train=20et=20aujourd'hui=20au?=
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+[[!meta title="Sensitive, multiplex and direct quantification of RNA sequences using a modified RASL assay."]]
+[[!tag method ligase transcript_count expression]]
+
+Larman HB, Scott ER, Wogan M, Oliveira G, Torkamani A, Schultz PG
+
+Nucleic Acids Res. 2014 Oct 1;42(14):9146-57. doi: 10.1093/nar/gku636
+
+Sensitive, multiplex and direct quantification of RNA sequences using a modified RASL assay.
+
+[[!pmidi 25063296 desc="Decoy probes to reduce the number of reads for the most highly expressed genes.  Uses T4 Rnl2, which was superior in consistency to splintR.  Ligation validated by capillary sequencing.  When hybridizing oligonucleotides to a target to form a nicked double strand, annealing is more efficient if the 5′ end at the nick is not phosphorylated."]]
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2.47.3