From f22d585f00a1e24e2a7819e77e35c8c8a7572db7 Mon Sep 17 00:00:00 2001 From: Charles Plessy Date: Wed, 23 Aug 2017 09:03:42 +0900 Subject: [PATCH] vieux --- biblio/10632587.mdwn | 10 ++++++++++ biblio/12399444.mdwn | 10 ++++++++++ biblio/12636919.mdwn | 9 ++++++++- biblio/To_Do | 6 ------ 4 files changed, 28 insertions(+), 7 deletions(-) create mode 100644 biblio/10632587.mdwn create mode 100644 biblio/12399444.mdwn diff --git a/biblio/10632587.mdwn b/biblio/10632587.mdwn new file mode 100644 index 00000000..7338f2e3 --- /dev/null +++ b/biblio/10632587.mdwn @@ -0,0 +1,10 @@ +[[!meta title="Kv4.2 mRNA abundance and A-type K(+) current amplitude are linearly related in basal ganglia and basal forebrain neurons."]] +[[!tag single_cell qPCR reverse-transcriptase]] + +J Neurosci. 2000 Jan 15;20(2):579-88. + +Tkatch T, Baranauskas G, Surmeier DJ. + +Kv4.2 mRNA abundance and A-type K(+) current amplitude are linearly related in basal ganglia and basal forebrain neurons. + +[[!pmid 10632587 desc="Accute dissociation of slices before cell isolation. Variablility between RT lots? Evaluation of mRNA abundance through a serial dillution and callibration approach."]] diff --git a/biblio/12399444.mdwn b/biblio/12399444.mdwn new file mode 100644 index 00000000..bbc8e944 --- /dev/null +++ b/biblio/12399444.mdwn @@ -0,0 +1,10 @@ +[[!meta title="Identification of cell-specific messenger ribonucleic acids in oxytocinergic and vasopressinergic magnocellular neurons in rat supraoptic nucleus by single-cell differential hybridization."]] +[[!tag single_cell library]] + +Yamashita M, Glasgow E, Zhang BJ, Kusano K, Gainer H. + +Endocrinology. 2002 Nov;143(11):4464-76. doi:10.1210/en.2002-220516 + +Identification of cell-specific messenger ribonucleic acids in oxytocinergic and vasopressinergic magnocellular neurons in rat supraoptic nucleus by single-cell differential hybridization. + +[[!pmid 12399444 desc="Single cell cDNA library construction. 5' poly A added by terminal transferase."]] diff --git a/biblio/12636919.mdwn b/biblio/12636919.mdwn index 59fdd72b..22c6d0c7 100644 --- a/biblio/12636919.mdwn +++ b/biblio/12636919.mdwn @@ -1,3 +1,10 @@ [[!meta title="Single-cell transcript analysis of pancreas development."]] [[!tag single_cell PCR]] -[[!pmid desc="Single cell PCR with 2× poly T."]] + +Chiang MK, Melton DA. + +Dev Cell. 2003 Mar;4(3):383-93. + +Single-cell transcript analysis of pancreas development. + +[[!pmid 12636919 desc="Single cell PCR with 2× poly T."]] diff --git a/biblio/To_Do b/biblio/To_Do index ab2aa66c..6698258d 100644 --- a/biblio/To_Do +++ b/biblio/To_Do @@ -37,9 +37,6 @@ ggcacgcga/cc => C-box for dro hairy. 10537171 Full in situ random priming cDNA synthesis -12399449 [single cell] -Single cell cDNA library construction. 5' poly A added by terminal transferase. - 9883850 [amplification] 3 rounds of T7 RNA amplification (aRNA). @@ -177,9 +174,6 @@ Differential distribution of TEs in the promoters. In 4.5% of the studied genes, 15215383 [networks] 1) Defines coregulated groups. TFs from these groups are proposed regulators of the group. 2) Analyses the predicted promoters with transfac or jaspar. 3) Builds a network from the results of 1) and 2). -10632582 [single cell] [tracked] -Accute dissociatin of slices before cell isolation. Variablility between RT lots? Evaluation of mRNA abundance through a serial dillution and callibration approach. - 12934013 [networks] [tracked] Defines metagenes as a set of genes from different organisms, that are each other's reciprocal best blast hit. Focuces on the highly clustered components of a metagene coexpression network. -- 2.47.3