From ee69a2db8536e3702ad7dc31bbcb7fc989a13320 Mon Sep 17 00:00:00 2001 From: Charles Plessy Date: Mon, 19 Mar 2018 17:37:55 +0900 Subject: [PATCH] Biblio --- biblio/24922482.mdwn | 10 ++++++++++ 1 file changed, 10 insertions(+) create mode 100644 biblio/24922482.mdwn diff --git a/biblio/24922482.mdwn b/biblio/24922482.mdwn new file mode 100644 index 00000000..1e10e5c1 --- /dev/null +++ b/biblio/24922482.mdwn @@ -0,0 +1,10 @@ +[[!meta title="Capture and Amplification by Tailing and Switching (CATS). An ultrasensitive ligation-independent method for generation of DNA libraries for deep sequencing from picogram amounts of DNA and RNA."]] +[[!tag template_switching amplification method]] + +RNA Biol. 2014;11(7):817-28. doi:10.4161/rna.29304 + +Turchinovich A, Surowy H, Serva A, Zapatka M, Lichter P, Burwinkel B. + +Capture and Amplification by Tailing and Switching (CATS). An ultrasensitive ligation-independent method for generation of DNA libraries for deep sequencing from picogram amounts of DNA and RNA. + +[[!pmid 24922482 desc="Pre-treatment by T4 PNK was needed when using circulating plasma RNA, probably because of phosphate or cyclo-phosphates at the 3′ end (caused by RNA cleavage). RT primer concentration scaled down according to the RNA amoounts (0.1 µM for 1 ng and 0.1 nM for 5 pg). TSO concentration: 1 µM."]] -- 2.47.3