From ec6e5d06d3417d47b06e9471efb79271dc97e83a Mon Sep 17 00:00:00 2001 From: Charles Plessy Date: Thu, 21 Sep 2017 16:15:12 +0900 Subject: [PATCH] Old --- biblio/12503310.mdw | 10 ++++++++++ biblio/To_Do | 3 --- 2 files changed, 10 insertions(+), 3 deletions(-) create mode 100644 biblio/12503310.mdw diff --git a/biblio/12503310.mdw b/biblio/12503310.mdw new file mode 100644 index 00000000..c28814e2 --- /dev/null +++ b/biblio/12503310.mdw @@ -0,0 +1,10 @@ +[[!meta title="Purification of histidine-tagged T4 RNA ligase from E. coli."]] +[[!tag enzyme ligase]] + +Biotechniques. 2002 Dec;33(6):1256-60 + +Wang QS, Unrau PJ. + +Purification of histidine-tagged T4 RNA ligase from E. coli. + +[[!pmid 12503310 desc="De-adenylation of T4 RNA ligase is required prior using adenylated substrates. Incubating with pyrophosphate during some purification steps does the job. Commercial T4 usually contains mostly de-adenylated enzyme. Mesuring reaction efficiency without ATP gives adenylation levels."]] diff --git a/biblio/To_Do b/biblio/To_Do index 672d15ef..9f1d5162 100644 --- a/biblio/To_Do +++ b/biblio/To_Do @@ -89,9 +89,6 @@ Adenylated RNA is a better substrate for T4 RNA ligase. 3299268 [enzymes] Synthesis of adenylated RNA -12503310 [enzymes] -De-adenylation of T4 RNA ligase is required prior using adenylated substrates. Incubating with pyrophosphate during some purification steps does the job. Commercial T4 usually contains mostly de-adenylated enzyme. Mesuring reaction efficiency without ATP gives adenylation levels. - 9373199 [libraries] Oligo-capping to build 5' ou FL cDNA libraries. -- 2.47.3