From e935a114d584722a24639d5c510a13a9ab1f1d2f Mon Sep 17 00:00:00 2001 From: Charles Plessy Date: Tue, 30 May 2017 10:12:56 +0900 Subject: [PATCH] Au bureau. --- biblio/28526029.mdwn | 10 ++++++++++ 1 file changed, 10 insertions(+) create mode 100644 biblio/28526029.mdwn diff --git a/biblio/28526029.mdwn b/biblio/28526029.mdwn new file mode 100644 index 00000000..08b22aef --- /dev/null +++ b/biblio/28526029.mdwn @@ -0,0 +1,10 @@ +[[!meta title="Cell fixation and preservation for droplet-based single-cell transcriptomics."]] +[[!tag methanol fixation method single_cell]] + +Alles J, Karaiskos N, Praktiknjo SD, Grosswendt S, Wahle P, Ruffault PL, Ayoub S, Schreyer L, Boltengagen A, Birchmeier C, Zinzen R, Kocks C, Rajewsky N. + +BMC Biol. 2017 May 19;15(1):44. doi:10.1186/s12915-017-0383-5 + +Cell fixation and preservation for droplet-based single-cell transcriptomics. + +[[!pmid 28526029 desc='"For rehydration, cells were either kept on ice after fixation (Fixed) or moved from –80 °C to 4 °C (Fixed 1 or 3 weeks) and kept in the cold throughout the procedure. Cells were pelleted at 1000 to 3000 × g, resuspended in PBS + 0.01% BSA, centrifuged again, resuspended in PBS + 0.01% BSA, passed through a 40- or 35-μm cell strainer, counted and diluted for Drop-seq in PBS + 0.01% BSA as described above. "']] -- 2.47.3