From e622a5d3f8c6c2f3ada81b816ff50a6a320e8619 Mon Sep 17 00:00:00 2001 From: Charles Plessy Date: Fri, 13 Oct 2017 15:36:00 +0900 Subject: [PATCH] Old --- biblio/16131588.mdwn | 10 ++++++++++ biblio/To_Do | 3 --- tags/emulsion.mdwn | 1 + 3 files changed, 11 insertions(+), 3 deletions(-) create mode 100644 biblio/16131588.mdwn diff --git a/biblio/16131588.mdwn b/biblio/16131588.mdwn new file mode 100644 index 00000000..6f5f73fd --- /dev/null +++ b/biblio/16131588.mdwn @@ -0,0 +1,10 @@ +[[!meta title="Direct selection of trans-acting ligase ribozymes by in vitro compartmentalization."]] +[[!tag emulsion biotin selection ribozyme]] + +RNA. 2005 Oct;11(10):1555-62. doi:10.1261/rna.2121705 + +Levy M, Griswold KE, Ellington AD. + +Direct selection of trans-acting ligase ribozymes by in vitro compartmentalization. + +[[!pmid 16131588 desc="During immunolabelling, biotinylated nucleic acids can diffuse from bead to bead if no blocking of biotin is added."]] diff --git a/biblio/To_Do b/biblio/To_Do index 2bb9c61c..4984443b 100644 --- a/biblio/To_Do +++ b/biblio/To_Do @@ -589,9 +589,6 @@ As tyramide is converted to a free radical which reacts with neighbouring protei 12433997 [emulsion] Random primers were used to introduce mutations. -16131588 [emulsion] -During immunolabelling, biotinylated nucleic acids can diffuse from bead to bead in no excess of biotin is added. - 11274352 [emulsion] No beads. A bacterial cel is used as a subcompartment maintaining gene and proteins together. Evidence for leakage of small molecules at high compartment density. diff --git a/tags/emulsion.mdwn b/tags/emulsion.mdwn index b2634774..65071cf8 100644 --- a/tags/emulsion.mdwn +++ b/tags/emulsion.mdwn @@ -11,6 +11,7 @@ How to break the droplets ? - High salt: - In [[Dielh _et al._, 2006|biblio/16791214]], by pipetting up and down in 100 mM NaCl, 1 % SDS, 1 % TritonX-100 and Tris (oil: mineral, superfactant: ABIL WE09). - In [[Kojima _et al._, 2005|biblio/16214800]], by mixing with 1M NaCl, 5 mM Tris-HCl pH 8.0, 0.5 mM EDTA (oil: mineral; superfactant: Sun Soft No. 818SK). + - Extraction of beads with hexane:oil 1:1, in [[Levy _et al._, 2005|biblio/16131588]] (oil: mineral, superfactant: 5.5% Span 80, 0.5% Tween 80, 0.1 % TritonX-100). Different kinds of superfactants: TritonX-100, Span 80, Tween 80 (many articles), ABIL WE09 ([[Dielh _et al._, 2006|biblio/16791214]] and others), Sun Soft No. 818SK (polyglycerol esters of intersesterified ricinoleic acid [[Kojima _et al._, 2005|biblio/16214800]]). -- 2.47.3