From e0f351b904d3038731ea394b5716459185d6ebbd Mon Sep 17 00:00:00 2001 From: Charles Date: Sun, 10 Mar 2019 10:44:06 +0900 Subject: [PATCH] CAPsmart --- biblio/25003736.mdwn | 2 +- tags/cap.mdwn | 7 +++++++ 2 files changed, 8 insertions(+), 1 deletion(-) diff --git a/biblio/25003736.mdwn b/biblio/25003736.mdwn index 878bb0fe..6f726400 100644 --- a/biblio/25003736.mdwn +++ b/biblio/25003736.mdwn @@ -1,5 +1,5 @@ [[!meta title="Four methods of preparing mRNA 5' end libraries using the illumina sequencing platform."]] -[[!tag template_switching method oligo-capping benchmark]] +[[!tag cap template_switching method oligo-capping benchmark]] Machida RJ, Lin YY. diff --git a/tags/cap.mdwn b/tags/cap.mdwn index 34d054e4..8e2ae7f3 100644 --- a/tags/cap.mdwn +++ b/tags/cap.mdwn @@ -56,6 +56,13 @@ in the [[template_switching]] tag page. - [[Kwak et al., 2013|biblio/23430654]] modified oligo-capping to create Pro-cap, a method for nuclear run-on analysis at single-nucleotide resulution. + - CapSMART ([[Machida and Lin, 2014|biblio/25003736]]): non-capped RNAs are + dephosphorylated, rephoshporylated and a blocking linker containing + isoguanosines and isocytidines (so that it can not be reverse-transcribed) is + ligated to them. A classical SMART protocol follows. cDNAs are amplified with + biotinylated forward primers, so that the 5′ end can be recovered after + mechanical fragmentation. + Atypical caps (work in progress) -------------------------------- -- 2.47.3