From dab30558fe64feeb10dffab51e6c499dcee6dac6 Mon Sep 17 00:00:00 2001 From: Charles Date: Tue, 4 Feb 2020 15:11:36 +0900 Subject: [PATCH] =?utf8?q?Caf=C3=A9?= MIME-Version: 1.0 Content-Type: text/plain; charset=utf8 Content-Transfer-Encoding: 8bit --- biblio/31719208.mdwn | 10 ++++++++++ 1 file changed, 10 insertions(+) create mode 100644 biblio/31719208.mdwn diff --git a/biblio/31719208.mdwn b/biblio/31719208.mdwn new file mode 100644 index 00000000..00673cc8 --- /dev/null +++ b/biblio/31719208.mdwn @@ -0,0 +1,10 @@ +[[!meta title="Definitive demonstration by synthesis of genome annotation completeness."]] +[[!tag synthethic]] + +Jaschke PR, Dotson GA, Hung KS, Liu D, Endy D. + +Proc Natl Acad Sci U S A. 2019 Nov 26;116(48):24206-24213. doi:10.1073/pnas.1905990116 + +Definitive demonstration by synthesis of genome annotation completeness. + +[[!pmid 31719208 desc="A øX174 genome where most cryptic ORFs were erased is still viable. In its initial version, its growth was slower than the wild type, but an in vitro selection isolated a point mutant that recovered the growth phenotype. Additional experiments suggest that the point mutation affected the translation levels of one of the major ORFs. A øX174 genome where the major ORFs are mutated is not viable, and only a single cryptic ORF shows detectable levels of expression."]] -- 2.47.3