From d6865665a12e8001f96fa96d8dc431c9fe26de1e Mon Sep 17 00:00:00 2001 From: Charles Plessy Date: Thu, 13 Dec 2018 15:23:49 +0900 Subject: [PATCH] Adapter sequences. --- biblio/18987734.mdwn | 7 +++++++ tags/sequencing.mdwn | 19 +++++++++++++++++-- 2 files changed, 24 insertions(+), 2 deletions(-) diff --git a/biblio/18987734.mdwn b/biblio/18987734.mdwn index 0509d3d5..243cf0a6 100644 --- a/biblio/18987734.mdwn +++ b/biblio/18987734.mdwn @@ -1,3 +1,10 @@ [[!meta title="Accurate whole human genome sequencing using reversible terminator chemistry."]] [[!tag method sequencing sequence_tags]] + +Nature. 2008 Nov 6;456(7218):53-9. doi: 10.1038/nature07517 + +Bentley DR, many co-authors, and Smith AJ. + +Accurate whole human genome sequencing using reversible terminator chemistry. + [[!pmid 18987734 desc="Primary paper for Illumina-Solexa technology. The supplemental information contains the sequence of the oligonucleotides used on the flow cells."]] diff --git a/tags/sequencing.mdwn b/tags/sequencing.mdwn index dcf670dc..ac52df52 100644 --- a/tags/sequencing.mdwn +++ b/tags/sequencing.mdwn @@ -1,4 +1,19 @@ [[!meta title="pages tagged sequencing"]] -[[!inline pages="tagged(sequencing)" actions="no" archive="yes" -feedshow=10]] +Illumina flowcell adaptors +-------------------------- + +Information extracted from the supplemental information of [[Bentley et al., 2008|bilbio/18987734]] (Accurate whole human genome sequencing using reversible terminator chemistry, Nature *456*(7218):53–9) + +Single read flowcell + + 5´-PSTTTTTTTTTT-(diol)3-AATGATACGGCGACCACCGA-3´ + 5´-PSTTTTTTTTTTCAAGCAGAAGACGGCATACGA-3´ + +Paired read flowcell + + 5´-PS-TTTTTTTTTTAATGATACGGCGACCACCGAGAUCTACAC-3´ (U = 2-deoxyuridine) + 5´-PS-TTTTTTTTTTCAAGCAGAAGACGGCATACGAGoxoAT-3´  (Goxo = 8-oxoguanine) + + +[[!inline pages="tagged(sequencing)" actions="no" limit=0]] -- 2.47.3