From d2a54b24439e71539a67155dfabd927b5b2d1b7e Mon Sep 17 00:00:00 2001 From: Charles Plessy Date: Wed, 1 Nov 2017 13:11:41 +0900 Subject: [PATCH] Old --- biblio/163011.mdwn | 10 ++++++++++ biblio/9373149.mdwn | 10 ++++++++++ biblio/To_Do | 3 --- tags/cap.mdwn | 5 +++++ 4 files changed, 25 insertions(+), 3 deletions(-) create mode 100644 biblio/163011.mdwn create mode 100644 biblio/9373149.mdwn diff --git a/biblio/163011.mdwn b/biblio/163011.mdwn new file mode 100644 index 00000000..c0853f99 --- /dev/null +++ b/biblio/163011.mdwn @@ -0,0 +1,10 @@ +[[!meta title="A blocked structure at the 5' terminus of mRNA from cytoplasmic polyhedrosis virus."]] +[[!tag cap not_read]] + +Nature. 1975 Jan 31;253(5490):374-5 + +Furuichi Y, Miura K. + +A blocked structure at the 5' terminus of mRNA from cytoplasmic polyhedrosis virus. + +[[!pmid 163011 desc="Discovery of the cap."]] diff --git a/biblio/9373149.mdwn b/biblio/9373149.mdwn new file mode 100644 index 00000000..eff393a4 --- /dev/null +++ b/biblio/9373149.mdwn @@ -0,0 +1,10 @@ +[[!meta title="Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library."]] +[[!tag cap library method]] + +Gene. 1997 Oct 24;200(1-2):149-56. + +Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S. + +Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library. + +[[!pmid 9373149 desc="Oligo-capping to build 5′ or FL cDNA libraries."]] diff --git a/biblio/To_Do b/biblio/To_Do index 8db5444d..8028f58c 100644 --- a/biblio/To_Do +++ b/biblio/To_Do @@ -74,9 +74,6 @@ Advocates a systematic round of RNA amplificatin, becaus it reduces interarray v T4 RNA ligase reaction efficiency improved by PEG and pre-adenylation. direct labelling method for big quantities: RNA fragmentation, then T4 RNA ligation of biotinylated oligos. -9373199 [libraries] -Oligo-capping to build 5' ou FL cDNA libraries. - 14606961 [libraries] The nuclease activity of T7 RNA pol could also show some transcript specificity. diff --git a/tags/cap.mdwn b/tags/cap.mdwn index 1ca3e1f3..27bfd583 100644 --- a/tags/cap.mdwn +++ b/tags/cap.mdwn @@ -6,6 +6,11 @@ Methods for enriching capped RNAs. - A method similar to oligo-capping was reported by [[Sekine and Kato|biblio/8247743]] in 1993. + - Oligo-capping [[Suzuki et al., 1999|biblio/9373149]]: dephosphorylate, uncover functional + phosphate with decapping enzyme, and ligate RNA adaptor with T4 RNA ligase. + +(wip: Fromont-racine et al., Maruyama et al.) + - CapSelect [[Schmidt et al., 1999|biblio/10518626]]: Add a poly-A tail to the first-strand cDNA, ligate a double-stranded adapter ending with a single-strand (T)TTTGGG overhang, and prime second-strand synthesis. -- 2.47.3