From cfcd44da61ae44478e173b01ee6fdbdb3e89e917 Mon Sep 17 00:00:00 2001 From: Charles Plessy Date: Mon, 11 Jun 2018 12:42:14 +0900 Subject: [PATCH] =?utf8?q?Avant=20le=20caf=C3=A9?= MIME-Version: 1.0 Content-Type: text/plain; charset=utf8 Content-Transfer-Encoding: 8bit --- biblio/29734295.mdwn | 10 ++++++++++ 1 file changed, 10 insertions(+) create mode 100644 biblio/29734295.mdwn diff --git a/biblio/29734295.mdwn b/biblio/29734295.mdwn new file mode 100644 index 00000000..9ce057a7 --- /dev/null +++ b/biblio/29734295.mdwn @@ -0,0 +1,10 @@ +[[!meta title="Genome-scale engineering of Saccharomyces cerevisiae with single-nucleotide precision."]] +[[!tag CRISPR method screen yeast]] + +Nat Biotechnol. 2018 Jul;36(6):505-508. doi:10.1038/nbt.4132 + +Bao Z, HamediRad M, Xue P, Xiao H, Tasan I, Chao R, Liang J, Zhao H. + +Genome-scale engineering of Saccharomyces cerevisiae with single-nucleotide precision. + +[[!pmid 29734295 desc="CRISPR–Cas9- and homology-directed-repair (HDR)-assisted genome-scale engineering (CHAnGE) uses plasmids containing HR arms to frameshift or point-mutate precise locations, which are cleaved by Cas9 using a guide RNA expressed by the same plasmid. Plasmid libraries are made by cloning libraries of long oligonucleotides synthesised on microarrays."]] -- 2.47.3