From ca577f93ca6412a06b1b1d33139e72313d906150 Mon Sep 17 00:00:00 2001
From: Charles Plessy <charles.plessy@oist.jp>
Date: Fri, 8 Feb 2019 16:31:20 +0900
Subject: [PATCH] Specificity of RT priming at higher temperatures.

---
 tags/reverse_transcription.mdwn | 3 +++
 1 file changed, 3 insertions(+)

diff --git a/tags/reverse_transcription.mdwn b/tags/reverse_transcription.mdwn
index 9e31aa18..c73e930f 100644
--- a/tags/reverse_transcription.mdwn
+++ b/tags/reverse_transcription.mdwn
@@ -89,6 +89,9 @@ nucleotide as a template.
  - Reported by [[Mizuno et al., 1999|biblio/9973624]].
  - Utilised in [[Arnaud et al., 2016|biblio/27071605]] to reduce priming or
    ribosomal or hemoglobin RNA.
+ - Specificity of RT nevertheless increases with reaction temperature.  For
+   instance, [[Xu and coll., 2019|biblio/30573814]] increased it from
+   ~30 % at 47 °C to ~75 % at 55 °C.
 
 ### Reverse-transcription primers
 
-- 
2.47.3