From c9681a305d5f1ea508dfec4aafcadece44fdbffe Mon Sep 17 00:00:00 2001 From: Charles Date: Fri, 18 Jun 2021 20:29:23 +0900 Subject: [PATCH] Avec du vin --- biblio/34099548.mdwn | 10 ++++++++++ tags/amplification.mdwn | 5 +++++ 2 files changed, 15 insertions(+) create mode 100644 biblio/34099548.mdwn diff --git a/biblio/34099548.mdwn b/biblio/34099548.mdwn new file mode 100644 index 00000000..ea8bcc35 --- /dev/null +++ b/biblio/34099548.mdwn @@ -0,0 +1,10 @@ +[[!meta title="Accurate genomic variant detection in single cells with primary template-directed amplification."]] +[[!tag amplification method]] + +Gonzalez-Pena V, Natarajan S, Xia Y, Klein D, Carter R, Pang Y, Shaner B, Annu K, Putnam D, Chen W, Connelly J, Pruett-Miller S, Chen X, Easton J, Gawad C. + +Proc Natl Acad Sci U S A. 2021 Jun 15;118(24):e2024176118. doi:10.1073/pnas.2024176118 + +Accurate genomic variant detection in single cells with primary template-directed amplification. + +[[!pmid 34099548 desc="“primary template-directed amplification (PTA) [...] takes advantage of the processivity, strong strand displacement activity, and low error rate of phi29 polymerase [...] exonuclease-resistant terminators are incorporated into the reaction, creating smaller double-stranded amplification products that undergo limited subsequent amplification. This transforms the reaction from exponential into a quasilinear process with more of the amplification occurring from the primary template.”"]] diff --git a/tags/amplification.mdwn b/tags/amplification.mdwn index 798fcbaf..7c8520fe 100644 --- a/tags/amplification.mdwn +++ b/tags/amplification.mdwn @@ -18,4 +18,9 @@ concentration and annealing temperature) were investigated by [[Dai and coll., complex sample, relatively long ITR (compared to primer length), high [annealing temperature], and high concentration of primer should be used.”. +## Other methods + + - Primary Template-directed Amplification (PTA), a kind of MDA with terminators + to limit the size of intermediate amplicaons [[Gonzalez-Pena and coll., 2021|biblio/34099548]] + [[!inline pages="tagged(amplification)" limit=0]] -- 2.47.3