From c895e54c209f098d2ea80c57b127cc6664cd6691 Mon Sep 17 00:00:00 2001 From: Charles Plessy Date: Wed, 9 Feb 2011 17:22:35 +0900 Subject: [PATCH] # Template switching. --- biblio/20876692.mdwn | 3 +++ 1 file changed, 3 insertions(+) create mode 100644 biblio/20876692.mdwn diff --git a/biblio/20876692.mdwn b/biblio/20876692.mdwn new file mode 100644 index 00000000..27bb1395 --- /dev/null +++ b/biblio/20876692.mdwn @@ -0,0 +1,3 @@ +[[!meta title="Reverse transcriptases can clamp together nucleic acids strands with two complementary bases at their 3'-termini for initiating DNA synthesis."]] +[[!tag template_switching enzyme manganese]] +[[!pmid 20876692 desc="AT-rich cDNA tails are not triggering template-switching well. CC are the strongest. Mn₂⁺ potentiates template switching for MMLV. dGTP stabilises the clamp between the enzyme and the nucleic acids."]] -- 2.47.3