From c6ae479f0d889e3fd91a933923478878ffefe135 Mon Sep 17 00:00:00 2001 From: Charles Date: Tue, 16 Jul 2019 13:13:16 +0900 Subject: [PATCH] Karyotyping in Ciona --- biblio/14993826.mdwn | 10 ++++++++++ 1 file changed, 10 insertions(+) create mode 100644 biblio/14993826.mdwn diff --git a/biblio/14993826.mdwn b/biblio/14993826.mdwn new file mode 100644 index 00000000..a33525b8 --- /dev/null +++ b/biblio/14993826.mdwn @@ -0,0 +1,10 @@ +[[!meta title="Fluorescent in situ hybridization to ascidian chromosomes."]] +[[!tag Ciona chromosome]] + +Zoolog Sci. 2004 Feb;21(2):153-7 doi:10.2108/zsj.21.153 + +Shoguchi E1, Ikuta T, Yoshizaki F, Satou Y, Satoh N, Asano K, Saiga H, Nishikata T. + +Fluorescent in situ hybridization to ascidian chromosomes. + +[[!pmid 14993826 desc="“Embryos at three different developmental stages (32-cell embryos, 64-cell embryos and gastrulae) were used to produce metaphase spreads, although treatment of 32-cell embryos and 64-cell embryos with 0.05% colchicine (Sigma) in seawater caused aggregated and disorganized chromosomes. After treatment with colchicine for 20–30 min, embryos were transferred to a 1.5-ml microfuge tube, to which cold methanol:glacial acetic acid (3:1) fixative was added (chilled on ice). The fixative was changed twice over a period of 1 hr and once after overnight (embryos kept at 4°C). The specimens were then stored in fixative at 4°C.”"]] -- 2.47.3