From aa22f6ef1fda5bf03922a7e1e31d44ff5e2ffd02 Mon Sep 17 00:00:00 2001 From: Charles Date: Thu, 9 Jan 2020 14:04:13 +0900 Subject: [PATCH] =?utf8?q?Ca=C6=92=C6=92=C3=A9?= MIME-Version: 1.0 Content-Type: text/plain; charset=utf8 Content-Transfer-Encoding: 8bit --- biblio/31862872.mdwn | 12 ++++++++++++ 1 file changed, 12 insertions(+) create mode 100644 biblio/31862872.mdwn diff --git a/biblio/31862872.mdwn b/biblio/31862872.mdwn new file mode 100644 index 00000000..b3e71f95 --- /dev/null +++ b/biblio/31862872.mdwn @@ -0,0 +1,12 @@ +[[!meta title="Novel approach reveals genomic landscapes of single-strand DNA breaks with nucleotide resolution in human cells."]] +[[!tag Helicos method]] + +Cao H, Salazar-García L, Gao F, Wahlestedt T, Wu CL, Han X, Cai Y, Xu D, Wang +F, Tang L, Ricciardi N, Cai D, Wang H, Chin MPS, Timmons JA, Wahlestedt C, +Kapranov P. + +Nat Commun. 2019 Dec 20;10(1):5799. doi:10.1038/s41467-019-13602-7 + +Novel approach reveals genomic landscapes of single-strand DNA breaks with nucleotide resolution in human cells. + +[[!pmid 31862872 desc="SSiNGLe: “single-strand break mapping at nucleotide genome level”. In the simplest form of this method, 3′-OH breaks are poly-A tailed and sequenced on a SeqLL (ex-Helicos) platform. A more complicated Illumina version exists. The authors found more breaks in gene regions (promoters, exons, introns), an also in mitochondrial DNA of aged patients and in regions displaying more sequence variants in the human population."]] -- 2.47.3