From a8d691e143ee5aeb42debba8df002b4ed52032e2 Mon Sep 17 00:00:00 2001 From: Charles Date: Thu, 16 Jul 2020 13:01:25 +0900 Subject: [PATCH] =?utf8?q?Caf=C3=A9?= MIME-Version: 1.0 Content-Type: text/plain; charset=utf8 Content-Transfer-Encoding: 8bit --- biblio/31036053.mdwn | 10 ++++++++++ 1 file changed, 10 insertions(+) create mode 100644 biblio/31036053.mdwn diff --git a/biblio/31036053.mdwn b/biblio/31036053.mdwn new file mode 100644 index 00000000..f1b6c7c3 --- /dev/null +++ b/biblio/31036053.mdwn @@ -0,0 +1,10 @@ +[[!meta title=""]] +[[!tag sequence_tag bias]] + +Measuring sequencer size bias using REcount: a novel method for highly accurate Illumina sequencing-based quantification. + +Gohl DM, Magli A, Garbe J, Becker A, Johnson DM, Anderson S, Auch B, Billstein B, Froehling E, McDevitt SL, Beckman KB. + +Genome Biol. 2019 Apr 29;20(1):85. doi:10.1186/s13059-019-1691-6 + +[[!pmid 31036053 desc="Sequencing templates already containing full Illumina adapters are cleaved from plasmids with a type IIS enzyme that generates blunt ends. Comparison between fixed-length and variable-length payloads allowed to study the size bias in Illumina sequencers. NextSeq > iSeq > MiSeq > NovaSeq. A freeze-thaw cycle reduced the prevalence of the smallest templates (~150 bp)."]] -- 2.47.3