From a5deab5671f5be422a5b7c044b89354259a15a48 Mon Sep 17 00:00:00 2001 From: Charles Plessy Date: Thu, 27 Jul 2017 09:10:16 +0900 Subject: [PATCH] Long time ago. --- biblio/16629394.mdwn | 10 ++++++++++ biblio/To_Do | 3 --- 2 files changed, 10 insertions(+), 3 deletions(-) create mode 100644 biblio/16629394.mdwn diff --git a/biblio/16629394.mdwn b/biblio/16629394.mdwn new file mode 100644 index 00000000..1c097d5e --- /dev/null +++ b/biblio/16629394.mdwn @@ -0,0 +1,10 @@ +[[!meta title="Amplification and analysis of cDNA generated from a single cell by 5'-RACE: application to isolation of antibody heavy and light chain variable gene sequences from single B cells."]] +[[!tag RACE]] + +Biotechniques. 2006 Apr;40(4):469-70, 472, 474 + +Ozawa T, Kishi H, Muraguchi A. + +Amplification and analysis of cDNA generated from a single cell by 5'-RACE: application to isolation of antibody heavy and light chain variable gene sequences from single B cells. + +[[!pmid 16629394 desc="50 % success on B cells to amplify the Ig variable region with polyG tailing and nested PCR."]] diff --git a/biblio/To_Do b/biblio/To_Do index 0219090f..4bd092a1 100644 --- a/biblio/To_Do +++ b/biblio/To_Do @@ -932,9 +932,6 @@ Concentrations as high as 1 M or 2.5 M can strongly improve the yield. 16533910 [enhancers] The directionality of the CNEs is often conserved beween paralogous loci. -16629394 [single cells] -50 % success on B cells to amplify the Ig variable region with polyG tailing and nested PCR. - 16682450 [misc] The structure of the genetic code favours secondary structures in coding RNA. -- 2.47.3