From a3e475529f912901ed13cf2e6fa54bf6093f7666 Mon Sep 17 00:00:00 2001 From: Charles Plessy Date: Thu, 2 Nov 2017 11:02:09 +0900 Subject: [PATCH] Expand. --- biblio/11713326.mdwn | 7 +++++++ tags/cap.mdwn | 5 +++++ 2 files changed, 12 insertions(+) diff --git a/biblio/11713326.mdwn b/biblio/11713326.mdwn index 1a698d93..7a677af1 100644 --- a/biblio/11713326.mdwn +++ b/biblio/11713326.mdwn @@ -1,3 +1,10 @@ [[!meta title="Detection of the 5'-cap structure of messenger RNAs with the use of the cap-jumping approach."]] [[!tag cap method]] + +Efimov VA, Chakhmakhcheva OG, Archdeacon J, Fernandez JM, Fedorkin ON, Dorokhov YL, Atabekov JG.Efimov VA1, Chakhmakhcheva OG, Archdeacon J, Fernandez JM, Fedorkin ON, Dorokhov YL, Atabekov JG. + +Nucleic Acids Res. 2001 Nov 15;29(22):4751-9. + +Detection of the 5'-cap structure of messenger RNAs with the use of the cap-jumping approach. + [[!pmid 11713326 desc="Binds an oligonucleotide to the cap using its free diol group, and integrates its sequence to the full-length cDNA using template-switching."]] diff --git a/tags/cap.mdwn b/tags/cap.mdwn index 9a0c954a..206f8328 100644 --- a/tags/cap.mdwn +++ b/tags/cap.mdwn @@ -16,6 +16,11 @@ Methods for enriching capped RNAs. the first-strand cDNA, ligate a double-stranded adapter ending with a single-strand (T)TTTGGG overhang, and prime second-strand synthesis. + - Cap-jumping ([[Efimov et al., 2001|biblio/11713326]]): oxidize diols, + covalently bind a 3′-amine oligonucleotide. The [[reverse_transcriptase]] + ireads through the cap structure and chemical bond, and integrates the reverse + complement of the oligonucleotide to the first-strand cDNA. + - [[Cleptet et al., 2004|biblio/14704363]] modified oligo-capping, to use T4 DNA ligase and a double-stranded adapter with NNNNNN overhang instead of T4 RNA ligase and a single-stranded linker. -- 2.47.3