From a0bb5370bcb96c02b561cdaac07c5f519b31e97b Mon Sep 17 00:00:00 2001 From: Charles Date: Mon, 6 Jul 2020 14:00:38 +0900 Subject: [PATCH] Cleanup --- biblio/16790564.mdwn | 10 ++++++++++ biblio/To_Do | 6 ------ 2 files changed, 10 insertions(+), 6 deletions(-) create mode 100644 biblio/16790564.mdwn diff --git a/biblio/16790564.mdwn b/biblio/16790564.mdwn new file mode 100644 index 00000000..cb8160df --- /dev/null +++ b/biblio/16790564.mdwn @@ -0,0 +1,10 @@ +[[!meta title="Primer Extension Enrichment Reaction (PEER): A New Subtraction Method for Identification of Genetic Differences Between Biological Specimens"]] +[[!tag method protocol]] + +Ganova-Raeva L, Zhang X, Cao F, Fields H, Khudyakov Y. + +Nucleic Acids Res. 2006 Jun 21;34(11):e76. doi: 10.1093/nar/gkl391 + +Primer Extension Enrichment Reaction (PEER): A New Subtraction Method for Identification of Genetic Differences Between Biological Specimens + +[[!pmid 16790564 desc="Generates primers by MmeI cleavage of cDNA extremities, and inactivates those which are complementary to the driver library by extending them with ddNTP"]] diff --git a/biblio/To_Do b/biblio/To_Do index 8021e42b..99956f1d 100644 --- a/biblio/To_Do +++ b/biblio/To_Do @@ -512,9 +512,6 @@ rasiRNA, the drosophila piRNA, are 5'P and one of their 2' or 3' is not OH. They 16778056 [small RNAs] Its neighbors got pseudogenized too in humans. -16790564 [protocols] -Generates primers by MmeI cleavage of cDNA extremities, and inactivates those which are complementary to the driver library by extending them with ddNTP. - 16822854 [protocols] The fluorophore and the quencher are separated by the strand-displacement activity of the enzyme. @@ -833,9 +830,6 @@ Evidence for complex polyA minus mRNA. 18836037 [misc] [not read] Having a binding site is not always essential. -18829718 [enzymes] -[not read]De-adenylates at 35 ℃, and circularises at 65 ℃. - 18988746 [genome] [not read] Many chromosomal aberrations in ES cells. -- 2.47.3