From 9a1027f36318fe82763810322b172775379880c2 Mon Sep 17 00:00:00 2001 From: Charles Plessy Date: Fri, 2 Feb 2018 14:28:45 +0900 Subject: [PATCH] Old --- biblio/4609429.mdwn | 10 ++++++++++ biblio/To_Do | 3 --- tags/ligase.mdwn | 5 +++++ 3 files changed, 15 insertions(+), 3 deletions(-) create mode 100644 biblio/4609429.mdwn diff --git a/biblio/4609429.mdwn b/biblio/4609429.mdwn new file mode 100644 index 00000000..33ec3246 --- /dev/null +++ b/biblio/4609429.mdwn @@ -0,0 +1,10 @@ +[[!meta title="T4 RNA ligase: substrate chain length requirements."]] +[[!tag ligase]] + +FEBS Lett. 1974 Sep 15;46(1):271-5. + +Kaufmann G, Klein T, Littauer UZ. + +T4 RNA ligase: substrate chain length requirements. + +[[!pmid 4609429 desc="For circularisation, (pA)8 is the shortest substrate, and the reaction is most efficient with (pA)10. The ligation efficicency then decreases with substrate length."]] diff --git a/biblio/To_Do b/biblio/To_Do index 68e22c22..ed99b9da 100644 --- a/biblio/To_Do +++ b/biblio/To_Do @@ -353,9 +353,6 @@ Out of 126 ultraconserved elements in a melanogaster / A. Gambiae comparison, on 7082652 [enzymes] T4RNL1 & AMP can remove or exchange 3' pNp from RNA molecles. -4609429 [enzymes] -(pA)8 is the smallet substrate, and the reaction is most efficient with (pA)10. The ligation efficicency then decreas with substrate length. - 12429865 [tags] Provides an easy access to various statistical tests. diff --git a/tags/ligase.mdwn b/tags/ligase.mdwn index d80e08ef..51c0ac02 100644 --- a/tags/ligase.mdwn +++ b/tags/ligase.mdwn @@ -2,6 +2,11 @@ Work in progress. +On T4 RNL1: + + - Using poly-A as substrate for circularisation, minimal length is 8, optimal + is 10, and then efficiency decreases slowly [[Kaufmann, Klein & Littauer, 1974|biblio/4609429]]. + On T4 RNL2: - Primary paper: [[Ho et al., 2002|biblio/12228725]]. -- 2.47.3