From 98b09c0787b2bf668ef23e4a658612d534c0633a Mon Sep 17 00:00:00 2001 From: Charles Plessy Date: Mon, 2 Mar 2020 16:08:43 +0900 Subject: [PATCH] Caps --- biblio/32103016.mdwn | 17 +++++++++++++++++ tags/cap.mdwn | 3 +++ 2 files changed, 20 insertions(+) create mode 100644 biblio/32103016.mdwn diff --git a/biblio/32103016.mdwn b/biblio/32103016.mdwn new file mode 100644 index 00000000..4aa7f3da --- /dev/null +++ b/biblio/32103016.mdwn @@ -0,0 +1,17 @@ +[[!meta title="Dinucleoside polyphosphates act as 5'-RNA caps in bacteria."]] +[[!tag cap]] + +Hudeček O, Benoni R, Reyes-Gutierrez PE, Culka M, Šanderová H, Hubálek M, Rulíšek L, Cvačka J, Krásný L, Cahová H. + +Nat Commun. 2020 Feb 26;11(1):1052. doi:10.1038/s41467-020-14896-8 + +Dinucleoside polyphosphates act as 5'-RNA caps in bacteria. + +[[!pmid 32103016 desc="T7 RNAP and E. coli RNAP efficiently initiate +transcription with N(p)nN cap analogs at mM concentrations. LC-MS analysis of +sRNAs digested with Nuclease P1 detects N(p)nN caps. Existence of the internal +polyphosphate chain demonstrated by the fact that the N(p)nN caps are not +fragmented by ionization, like GppppG and unlike pppGpG. m7Gp4Gm and m6Ap3A +were further identified using synthethic standards. The RppH and ApaH enzymes +cleaved caps, leaving 5′-p or 5′-ppp ends respectively. Cap methylation +protects from RppH cleavage but not from ApaH."]] diff --git a/tags/cap.mdwn b/tags/cap.mdwn index 8e2ae7f3..2b911981 100644 --- a/tags/cap.mdwn +++ b/tags/cap.mdwn @@ -69,4 +69,7 @@ Atypical caps (work in progress) - 5′-phospho-ADP-ribosylated RNA/DNA cap, synthethysed by RNA 2′-phosphotransferase Tpt1 ([[Munir, Banerjee and Shuman, 2018|biblio/30202863]]). + - Mass spectroscopy and molecular biology detected dinucleoside polyphosphate caps + in bacteria ([[Hudeček and coll., 2020|biblio/32103016]]). + [[!inline pages="tagged(cap)" limit=0]] -- 2.47.3