From 973330f93633de95d5cc35c6d8863b02f4aff652 Mon Sep 17 00:00:00 2001 From: Charles Plessy Date: Tue, 5 Nov 2013 17:51:46 +0900 Subject: [PATCH] =?utf8?q?Un=20papier,=20un=20caf=C3=A9.?= MIME-Version: 1.0 Content-Type: text/plain; charset=utf8 Content-Transfer-Encoding: 8bit --- biblio/24179226.mdwn | 10 ++++++++++ biblio/To_Do | 3 --- 2 files changed, 10 insertions(+), 3 deletions(-) create mode 100644 biblio/24179226.mdwn diff --git a/biblio/24179226.mdwn b/biblio/24179226.mdwn new file mode 100644 index 00000000..1adb6296 --- /dev/null +++ b/biblio/24179226.mdwn @@ -0,0 +1,10 @@ +[[!meta title="Mosaic copy number variation in human neurons."]] +[[!tag single_cell genome human]] + +McConnell MJ, Lindberg MR, Brennand KJ, Piper JC, Voet T, Cowing-Zitron C, Shumilina S, Lasken RS, Vermeesch JR, Hall IM, Gage FH. + +Science. 2013 Nov 1;342(6158):632-7. doi: 10.1126/science.1243472 + +Mosaic copy number variation in human neurons. + +[[!pmid 24179226 desc="Signal averaged over hundreds of kilobases to prevent from artefactual lapses of coverage."]] diff --git a/biblio/To_Do b/biblio/To_Do index 72c86a44..762a34c8 100644 --- a/biblio/To_Do +++ b/biblio/To_Do @@ -52,9 +52,6 @@ Aminoallyl UTP to labal cRNAs. In contrast to cDNAs, DMSO is required for subseq 11955710 [visual cortex] Cat visual cortex cDNAs hybridized to human macroarrays to study critical period mechanism -12636919 [single cell] -Single cell PCR, using 5'poly1-tagged cDNAs. - 11222780 [amplification] T7 pol generates aberrant template-unrelated products. This is fixed by reducing the concentration of oligo dT primers, and of enzymes. Under these conditions, 2 rounds of RNA linear amplification is not generatng too much bias. Small-volume protocol. T4gp32, a single stranded protein, increases RT processivity. -- 2.47.3