From 7ff17b22da19bc3acb103b88bdfb1f6b7e7a2fa0 Mon Sep 17 00:00:00 2001 From: Charles Date: Wed, 24 Apr 2019 15:07:49 +0900 Subject: [PATCH] RT clamping. --- biblio/20876692.mdwn | 2 +- tags/reverse_transcription.mdwn | 11 +++++++++++ 2 files changed, 12 insertions(+), 1 deletion(-) diff --git a/biblio/20876692.mdwn b/biblio/20876692.mdwn index a22c2390..5fae50be 100644 --- a/biblio/20876692.mdwn +++ b/biblio/20876692.mdwn @@ -1,5 +1,5 @@ [[!meta title="Reverse transcriptases can clamp together nucleic acids strands with two complementary bases at their 3'-termini for initiating DNA synthesis."]] -[[!tag template_switching enzyme manganese]] +[[!tag reverse_transcription template_switching enzyme manganese]] Oz-Gleenberg I, Herschhorn A, Hizi A. diff --git a/tags/reverse_transcription.mdwn b/tags/reverse_transcription.mdwn index 22f56036..8b290f13 100644 --- a/tags/reverse_transcription.mdwn +++ b/tags/reverse_transcription.mdwn @@ -11,6 +11,7 @@ _(redaction in progress)_ - [[T4 bacteriophage gene 32 protein|ssbp]] (T4gp32, [[Kenzelmann _et al._, 2004|biblio/15028277]], [[Piché _et al._, 2005|biblio/16461948]]). + ### DNA-dependent DNA polymerase activity - It is utilised in [[template_switching]] methods to add linkers to first-strand cDNAs. @@ -20,6 +21,16 @@ _(redaction in progress)_ artefacts ([[Perocchi et al., 2007|biblio/17897965]], [[Kanamori-Katayama et al., 2011|biblio/21596820]]). - Its error profile is different from other DNA polymerases ([[de Paz et al., 2018|biblio/29718339]]). + +### Clamping activity + + - Reverse transcriptases can bind duplexes that are only annealed through a + 2-nt homology. A single nucleotide is not enough and GC-rich sequences are + strongly favoured. This binding is enhanced by dGTP. The assays demonstrating + this used an ELISA approach ([[Oz-Gleenberg, Herschhorn and Hizi, + 2011|biblio/20876692]]). + + ### Terminal desoxynucleotidyl transferase (TdT) activity Like other DNA polymerases ([[Clark, 1988|biblio/2460825]]), reverse -- 2.47.3