From 7513b8e933fef5893d4e015d050ff1754e70bd51 Mon Sep 17 00:00:00 2001 From: Charles Plessy Date: Thu, 31 Oct 2013 17:17:06 +0900 Subject: [PATCH] =?utf8?q?Lu=20il=20y=20a=20tr=C3=A8s=20longtemps.?= MIME-Version: 1.0 Content-Type: text/plain; charset=utf8 Content-Transfer-Encoding: 8bit --- biblio/12089562.mdwn | 10 ++++++++++ biblio/To_Do | 6 ------ 2 files changed, 10 insertions(+), 6 deletions(-) create mode 100644 biblio/12089562.mdwn diff --git a/biblio/12089562.mdwn b/biblio/12089562.mdwn new file mode 100644 index 00000000..2e378cf5 --- /dev/null +++ b/biblio/12089562.mdwn @@ -0,0 +1,10 @@ +[[!meta title="Amine-modified random primers to label probes for DNA microarrays."]] +[[!tag microarray amplification method]] + +Xiang CC, Kozhich OA, Chen M, Inman JM, Phan QN, Chen Y, Brownstein MJ. + +Nat Biotechnol. 2002 Jul;20(7):738-42. + +Amine-modified random primers to label probes for DNA microarrays. + +[[!pmid desc="Incorporates aminoallyl-dUTP instead of cy3/5dUTP. Uses priming hexamers with a amino C6dT in 5′. This allows to reduce by 10 folds the quantity of RNA as a starting material. Presence of amplification products of t/rRNAs is not a problem when « optimal » quantities of total RNA are used."]] diff --git a/biblio/To_Do b/biblio/To_Do index e8babc7b..72c86a44 100644 --- a/biblio/To_Do +++ b/biblio/To_Do @@ -22,12 +22,6 @@ Adds a second round of amplification 11687821 Review citing the 2 upper articles -12089562 [amplification] -* Incorporates aminoallyl-dUTP instead of cy3/5dUTP -* uses priming hexamers with a amino C6dT in 5' -This allows to reduce by 10 folds the quantity of RNA as a starting material. -* Presence of amplification products of t/rRNAs is not a problem when «optimal» quantities of total RNA are used. - 12172558 * Says that exponential (65+25 cycles) is more faithful than linear. * Limits [dNTP] to produce only 3' cDNA that are a few hundrer base pairs long. -- 2.47.3