From 6ec69a16f0b5c68a973d054cf10c66540cac078b Mon Sep 17 00:00:00 2001 From: Charles Date: Tue, 27 Aug 2019 14:07:32 +0900 Subject: [PATCH] =?utf8?q?Caf=C3=A9?= MIME-Version: 1.0 Content-Type: text/plain; charset=utf8 Content-Transfer-Encoding: 8bit --- biblio/29741723.mdwn | 11 +++++++++++ tags/ligase.mdwn | 9 +++++++++ 2 files changed, 20 insertions(+) create mode 100644 biblio/29741723.mdwn diff --git a/biblio/29741723.mdwn b/biblio/29741723.mdwn new file mode 100644 index 00000000..a752a8b9 --- /dev/null +++ b/biblio/29741723.mdwn @@ -0,0 +1,11 @@ +[[!meta title="A single-molecule sequencing assay for the comprehensive profiling of T4 DNA ligase fidelity and bias during DNA end-joining."]] +[[!tag enzyme ligase]] + +Nucleic Acids Res. 2018 Jul 27;46(13):e79. doi:10.1093/nar/gky303 + +Potapov V, Ong JL, Langhorst BW, Bilotti K, Cahoon D, Canton B, Knight TF, +Evans TC Jr, Lohman GJS. + +A single-molecule sequencing assay for the comprehensive profiling of T4 DNA ligase fidelity and bias during DNA end-joining. + +[[!pmid 29741723 desc="TNA overhangs are very inefficient to ligate. However ANT are not harder to ligate than similar mismatches. GC-rich overhangs better tolerate mismatches than AT-rich ones. Increasing temperature reduced mismatched ligations. 5′-G mismatches are much better tolerated than 5′-T mismatches."]] diff --git a/tags/ligase.mdwn b/tags/ligase.mdwn index 2ba035e9..c05a18a6 100644 --- a/tags/ligase.mdwn +++ b/tags/ligase.mdwn @@ -51,6 +51,15 @@ DNA and RNA ligases can be used to adenylylate a substrate. - [[Torchia, Takagi and Ho (2008)|biblio/18829718]] reported the use of the Methanobacterium RNA ligase (MthRnl) to adenylylate RNA. +## Sequence biases + +(work in progress) + + - Potapov and coll. ([[2018|biblio/29741723]]) studied in details the tolerance + to mismatches of T4 DNA ligase when ligating 3' overhangs. Mismatches were + better tolerated with higher GC content and lower temperature. Surprisingly, + TNA overhangs were especially hard to ligate. + [[!inline pages="tagged(ligase)" limit=0]] [[!meta title="pages tagged adenylylation"]] -- 2.47.3