From 6d2bb1cbbe4b40094c140025dc5b4c2ae1507308 Mon Sep 17 00:00:00 2001 From: Charles Date: Mon, 2 Sep 2019 13:59:47 +0900 Subject: [PATCH] =?utf8?q?Caf=C3=A9?= MIME-Version: 1.0 Content-Type: text/plain; charset=utf8 Content-Transfer-Encoding: 8bit --- biblio/31114914.mdwn | 10 ++++++++++ tags/ligase.mdwn | 13 +++++++++---- 2 files changed, 19 insertions(+), 4 deletions(-) create mode 100644 biblio/31114914.mdwn diff --git a/biblio/31114914.mdwn b/biblio/31114914.mdwn new file mode 100644 index 00000000..6bc84b19 --- /dev/null +++ b/biblio/31114914.mdwn @@ -0,0 +1,10 @@ +[[!meta title="Highly efficient single-stranded DNA ligation technique improves low-input whole-genome bisulfite sequencing by post-bisulfite adaptor tagging."]] +[[!tag method ligase]] + +Nucleic Acids Res. 2019 May 22. pii: gkz435. doi:10.1093/nar/gkz435 + +Miura F, Shibata Y, Miura M, Sangatsuda Y, Hisano O, Araki H, Ito T. + +Highly efficient single-stranded DNA ligation technique improves low-input whole-genome bisulfite sequencing by post-bisulfite adaptor tagging. + +[[!pmid desc="Add a A-tail with TdT before ligation, because ligase prefers As."]] diff --git a/tags/ligase.mdwn b/tags/ligase.mdwn index c05a18a6..527c63a3 100644 --- a/tags/ligase.mdwn +++ b/tags/ligase.mdwn @@ -29,7 +29,7 @@ On T4 RNL2: linker to RNA ends, thus prevents unwanted ligation products ([[Viollet et al., 2011|biblio/21722378]]). -DNA and RNA ligases can be used to adenylylate a substrate. +DNA and RNA ligases can be used to [[adenylylate|adenylylation]] a substrate. - [[McLaughlin, Piel and Graeser (1985)|biblio/3978074]] noted that adenylylated RNA is a better substrate for T4 RNA ligase, and that @@ -60,8 +60,13 @@ DNA and RNA ligases can be used to adenylylate a substrate. better tolerated with higher GC content and lower temperature. Surprisingly, TNA overhangs were especially hard to ligate. -[[!inline pages="tagged(ligase)" limit=0]] -[[!meta title="pages tagged adenylylation"]] +## Methods using ligases: + +(works continuously in progress) + - TACS (terminal deoxyribonucleotidyl transferase (TdT)-assisted adenylate + connector-mediated ssDNA) tails DNA with As using the TdT, in order to + benefit from the ligase's preference for As ([[Miura and coll., + 2019|biblio/31114914]]). -[[!inline pages="tagged(adenylylation)" limit=0]] +[[!inline pages="tagged(ligase)" limit=0]] -- 2.47.3