From 6730f1a6627d57c2ae390f599f65d4d93285eb8c Mon Sep 17 00:00:00 2001 From: Charles Plessy Date: Thu, 15 Feb 2018 10:50:17 +0900 Subject: [PATCH] Old --- biblio/16707659.mdwn | 10 ++++++++++ biblio/To_Do | 3 --- tags/reverse_transcription.mdwn | 3 +++ tags/ssbp.mdwn | 8 ++++++-- 4 files changed, 19 insertions(+), 5 deletions(-) create mode 100644 biblio/16707659.mdwn diff --git a/biblio/16707659.mdwn b/biblio/16707659.mdwn new file mode 100644 index 00000000..96e5eae7 --- /dev/null +++ b/biblio/16707659.mdwn @@ -0,0 +1,10 @@ +[[!meta title="Improvements of rolling circle amplification (RCA) efficiency and accuracy using Thermus thermophilus SSB mutant protein."]] +[[!tag amplification method ssbp]] + +Inoue J, Shigemori Y, Mikawa T. + +Nucleic Acids Res. 2006 May 17;34(9):e69 doi:10.1093/nar/gkl350 + +Improvements of rolling circle amplification (RCA) efficiency and accuracy using Thermus thermophilus SSB mutant protein. + +[[!pmid 16707659 desc="By weakening the DNA-protein interaction, the authors acheived the suppression of the non-temlated products in rolling circle amplification."]] diff --git a/biblio/To_Do b/biblio/To_Do index dba387ff..af4f14a6 100644 --- a/biblio/To_Do +++ b/biblio/To_Do @@ -479,9 +479,6 @@ The directionality of the CNEs is often conserved beween paralogous loci. 16682450 [misc] The structure of the genetic code favours secondary structures in coding RNA. -16707659 [enzymes] -By weakening the DNA-protein interaction, the authors acheived the suppression of the non-temlated products in rolling circle amplification. - 16618967 [enzymes] The combined use of a complementary DNA oligo and DNA ligase suppresses the artifacts seen in RNA ligase adenylation. diff --git a/tags/reverse_transcription.mdwn b/tags/reverse_transcription.mdwn index 87e02af0..f6230ba7 100644 --- a/tags/reverse_transcription.mdwn +++ b/tags/reverse_transcription.mdwn @@ -24,4 +24,7 @@ Reverse-transcription primers: - "Not-so random" (NSR) primers: [[Armour et al., 2009|biblio/19668204]]. - ... and many more (pseudo-random, ...) +[[Single-strand binding proteins|ssbp]] are sometimes added to improve reverse +transcriptions. + [[!inline pages="tagged(reverse_transcription)" actions="no" limit=0]] diff --git a/tags/ssbp.mdwn b/tags/ssbp.mdwn index d841fb01..c00ebba5 100644 --- a/tags/ssbp.mdwn +++ b/tags/ssbp.mdwn @@ -1,4 +1,8 @@ [[!meta title="pages tagged ssbp"]] -[[!inline pages="tagged(ssbp)" actions="no" archive="yes" -feedshow=10]] +Single-strand binding proteins +============================== + +(Often used as additives in molecular biology reactions.) + +[[!inline pages="tagged(ssbp)" actions="no" limit=0]] -- 2.47.3