From 62a38801ccdfc09d3d8f021e37a5a0fcacd64447 Mon Sep 17 00:00:00 2001 From: Charles Plessy Date: Mon, 21 May 2018 16:47:20 +0900 Subject: [PATCH] Old --- tags/reverse_transcription.mdwn | 7 +++++++ 1 file changed, 7 insertions(+) diff --git a/tags/reverse_transcription.mdwn b/tags/reverse_transcription.mdwn index 5ccf4b15..a8ba77be 100644 --- a/tags/reverse_transcription.mdwn +++ b/tags/reverse_transcription.mdwn @@ -39,6 +39,7 @@ transcriptases have a TdT activity. transcriptase of the long terminal repeat retrotransposon Tf1, like other DNA polymerases, also adds non-templated As to blunt DNA duplexes. + ### Templated TdT activity Surprisingly, reverse transcriptases can also extend cDNAs using a single @@ -55,6 +56,12 @@ nucleotide as a template. 2017b|biblio/28747695]]). +### The 5′ cap enhances C-tailing + + - [[Schmidt & Mueller, 1999|biblio/10518626]] showed that extra cytosine are + more frequently added in presence of the 5′ cap. + + ### The 5′ cap is reverse-transcribed - [[Hirzmann et al., 1993|biblio/8346046]] observed the presence of an extra G -- 2.47.3