From 5bdad2efeef596f360419a16639de6918a04c29f Mon Sep 17 00:00:00 2001 From: Charles Plessy Date: Thu, 5 Oct 2017 09:22:25 +0900 Subject: [PATCH] Old --- biblio/15272081.mdwn | 10 ++++++++++ biblio/To_Do | 6 ------ 2 files changed, 10 insertions(+), 6 deletions(-) create mode 100644 biblio/15272081.mdwn diff --git a/biblio/15272081.mdwn b/biblio/15272081.mdwn new file mode 100644 index 00000000..95a7d699 --- /dev/null +++ b/biblio/15272081.mdwn @@ -0,0 +1,10 @@ +[[!meta title="5' Long serial analysis of gene expression (LongSAGE) and 3' LongSAGE for transcriptome characterization and genome annotation."]] +[[!tag sequence_tags library]] + +Proc Natl Acad Sci U S A. 2004 Aug 10;101(32):11701-6. doi:10.1073/pnas.0403514101 + +Wei CL, Ng P, Chiu KP, Wong CH, Ang CC, Lipovich L, Liu ET, Ruan Y. + +5' Long serial analysis of gene expression (LongSAGE) and 3' LongSAGE for transcriptome characterization and genome annotation. + +[[!pmid 15272081 desc="81% of the 5'/3' tag pairs could be used to amplify a cDNA by RT-PCR."]] diff --git a/biblio/To_Do b/biblio/To_Do index 524dacc7..88c9c563 100644 --- a/biblio/To_Do +++ b/biblio/To_Do @@ -89,9 +89,6 @@ T4 DNA ligase used to ligate a double stranded cap-tag. 3799962 [enzymes] Optimal : Donor/acceptor 5:1; hexammine cobalt chloride 5.0 mM, PEG8000 25%; ATP 100µM. Inhibitors: ATP > 100 µM, MN 2+ > 5mM, NaCl > 20 mM. -15247329 [libraries] [tracked] -Ligation of cDNA and methylated oligos, in presence of methy-sensitive restriction enzymes, eliminates the cDNA dimer product du to mass action. - 14513556 [libraries] Hybridises single-stranded tester plasmid and driver PCR product, then purified single strand molecules out of duplexes using hydroxyapatite. @@ -101,9 +98,6 @@ A method to amplify two distinguishable substrates in the same PCR tube, so that 12582258 [misc] Direct labeling of 10µg total RNA. -15272081 [libraries] -81% of the 5'/3' tag pairs could be used to amplify a cDNA by RT-PCR. - 8125298 [libraries] Oligo capping primary paper. -- 2.47.3