From 58d26c5c76c1b6b43efdbfc1e2166db1a31055f3 Mon Sep 17 00:00:00 2001 From: Charles Plessy Date: Tue, 20 Sep 2022 13:09:10 +0900 Subject: [PATCH] =?utf8?q?Caf=C3=A9?= MIME-Version: 1.0 Content-Type: text/plain; charset=utf8 Content-Transfer-Encoding: 8bit --- biblio/35880393.mdwn | 10 ++++++++++ tags/synthetic.mdwn | 6 ++++++ 2 files changed, 16 insertions(+) create mode 100644 biblio/35880393.mdwn diff --git a/biblio/35880393.mdwn b/biblio/35880393.mdwn new file mode 100644 index 00000000..65a32373 --- /dev/null +++ b/biblio/35880393.mdwn @@ -0,0 +1,10 @@ +[[!meta title="Production of retinoic acid by engineered Saccharomyces cerevisiae using an endogenous aldehyde dehydrogenase."]] +[[!tag synthetic yeast]] + +Hu Q, Yu H, Ye L. + +Biotechnol Bioeng. 2022 Jul 26. doi:10.1002/bit.28192 + +Production of retinoic acid by engineered Saccharomyces cerevisiae using an endogenous aldehyde dehydrogenase. + +[[!pmid 35880393 desc="Screen for the most efficient aldehyde dehydrogenases to produce retinoic acid in S. cerevisiae strain Y03 identified MmAldh, which was used to scan the yeast genome for an endogenous replacement. Hfd1 was found, and increasing its copy number lead to the best yields."]] diff --git a/tags/synthetic.mdwn b/tags/synthetic.mdwn index 009c3613..120fc94d 100644 --- a/tags/synthetic.mdwn +++ b/tags/synthetic.mdwn @@ -2,6 +2,8 @@ _in progress_ +## Genomes + Sc3.0 roadmap: [[Dai and coll., 2020|biblio/32791980]]. “Biosynthesis of medicinal tropane alkaloids in yeast”: [[Srinivasan and Smolke and coll., 2020|biblio/32879484]] @@ -10,4 +12,8 @@ Example of neochromosome synthesis in yeast: [[Postma and coll., 2021|biblio/334 Artificial genome scrambling in yeast 2.0 with loxPsym sites: [[Brooks and coll, 2022|biblio/35239377]]. +## Pathway + +Increasing the copy number of the endogenous aldehyde dehydrogenase Hfd1 allowed [[Hu, Yu and Ye, 2022|biblio/35880393]] to obtain the best yield of conversion of retinol to retinoic acid.. + [[!inline pages="tagged(synthethic)" limit=0]] -- 2.47.3