From 5384721115a0d0c50d3b5ca3c6991c7e619c283c Mon Sep 17 00:00:00 2001
From: Charles Plessy <https://launchpad.net/~plessy>
Date: Sun, 26 Aug 2012 10:35:26 +0900
Subject: [PATCH] One per day.

---
 biblio/8969838.mdwn | 10 ++++++++++
 1 file changed, 10 insertions(+)
 create mode 100644 biblio/8969838.mdwn

diff --git a/biblio/8969838.mdwn b/biblio/8969838.mdwn
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+[[!meta title="RT-PCR without RNA isolation."]]
+[[!tag RNA extraction RT-PCR DTT]]
+
+Klebe RJ, Grant GM, Grant AM, Garcia MA, Giambernardi TA, Taylor GP.
+
+Biotechniques. 1996 Dec;21(6):1094-100.
+
+RT-PCR without RNA isolation.
+
+[[!pmid 8969838 desc="Freeze-thaw method.  5 mM DTT partially protects the RNA for 1 h in absence of RNAse inhibitors.  Hypertonic solutions may prevent the premature disruption of the cell and its lysosomes prior lysis."]]
-- 
2.47.3