From 4c5a1ad90ad8d516ab6425b105466146b670f426 Mon Sep 17 00:00:00 2001 From: Charles Date: Mon, 27 Jan 2020 13:33:51 +0900 Subject: [PATCH] CapFinder. --- tags/template_switching.mdwn | 4 ++++ 1 file changed, 4 insertions(+) diff --git a/tags/template_switching.mdwn b/tags/template_switching.mdwn index 24ba14f9..d3bef696 100644 --- a/tags/template_switching.mdwn +++ b/tags/template_switching.mdwn @@ -2,6 +2,10 @@ (work in progress. For alternative cap enrichement methods see the [[cap]] tag page) + - 1996: ”Synthesis of high-quality cDNA from nanograms of total or polyA+ RNA + with the CapFinder PCR cDNA library construction kit.” Zhu, Y., A. Chenchik + and P.D. Siebert. CLONTECHniques 1 1:12-13. Could not find the PDF. + - In the "_CapSelect_" method, [[Schmidt and Mueller, 1999|biblio/10518626]] stimulate template switching with manganese (see below), tail the first-strand cDNAs with dA, and add 5′ linkers with T4 DNA ligase and duplex adapters -- 2.47.3