From 483e14e82e2627223587ef8179f2cee8f47e392e Mon Sep 17 00:00:00 2001 From: Charles Plessy Date: Wed, 15 Feb 2017 12:48:38 +0900 Subject: [PATCH] Lu dans le lit. --- biblio/22749402.mdwn | 13 +++++++++++++ 1 file changed, 13 insertions(+) create mode 100644 biblio/22749402.mdwn diff --git a/biblio/22749402.mdwn b/biblio/22749402.mdwn new file mode 100644 index 00000000..4239e793 --- /dev/null +++ b/biblio/22749402.mdwn @@ -0,0 +1,13 @@ +[[!meta title="Short structured RNAs with low GC content are selectively lost during extraction from a small number of cells."]] +[[!tag purification method Trizol miRNA bias]] + +Mol Cell. 2012 Jun 29;46(6):893-5. doi:10.1016/j.molcel.2012.05.036 + +Kim YK, Yeo J, Kim B, Ha M, Kim VN. + +Short structured RNAs with low GC content are selectively lost during extraction from a small number of cells. + +[[!pmid 22749402 desc="In extraction methods using Trizol, the long RNAs present in the cell lysate +act as carrier for the short RNAs, and when initial cell numbers are low or magnesium ions +are chelated, structured small RNAs become selectively harder to capture, which causes +a bias that can be confounded with key experimental parameters."]] -- 2.47.3