From 4698e285b4568d00fd68b047902b764dfcedfe4e Mon Sep 17 00:00:00 2001 From: admin Date: Mon, 15 Jun 2020 07:48:27 +0000 Subject: [PATCH] updated PO files --- open-source-biologist.en.po | 58 ++++++++++++++++++------------------- 1 file changed, 29 insertions(+), 29 deletions(-) diff --git a/open-source-biologist.en.po b/open-source-biologist.en.po index 16ff1771..20b07d75 100644 --- a/open-source-biologist.en.po +++ b/open-source-biologist.en.po @@ -7,7 +7,7 @@ msgid "" msgstr "" "Project-Id-Version: PACKAGE VERSION\n" -"POT-Creation-Date: 2020-06-15 07:45+0000\n" +"POT-Creation-Date: 2020-06-15 07:48+0000\n" "PO-Revision-Date: YEAR-MO-DA HO:MI+ZONE\n" "Last-Translator: FULL NAME \n" "Language-Team: LANGUAGE \n" @@ -23,30 +23,30 @@ msgstr "" #. type: Plain text msgid "" -"My training as a researcher started with developmental genetics in " -"drosophila and zebrafish, where I studied the activity of transcription " +"My training as a researcher started with **developmental genetics in " +"drosophila and zebrafish**, where I studied the activity of transcription " "enhancers ([Blader and coll., 2003](https://pubmed.gov/12559493)) and their " "evolutionary conservation (Plessy et al., 2005). This gave me a strong " "interest for whole-transcriptome analysis and technology. For that purpose, " "I have joined RIKEN in 2004, where have worked on high-throughput methods " -"for profiling promoters and inferring gene networks, and in particular on " -"CAGE (Cap Analysis Gene Expression)." +"for **profiling promoters and inferring gene networks**, and in particular " +"on CAGE (Cap Analysis Gene Expression)." msgstr "" #. type: Plain text msgid "" -"I have developed a miniaturized version of CAGE, termed nanoCAGE, to analyse " -"small samples yielding only nanograms of RNA (Plessy et al., 2010). In the " -"same manuscript, we also introduced its paired-end variant, CAGEscan, which " -"we use to associate novel promoters with annotations. Since then, we have " -"kept improving or expanding these techniques, by updating the protocol " -"(Salimullah et al., 2011), reducing the sequence bias introduced by the " -"molecular barcodes (Tang et al., 2013), combining multiple cap-enrichment " -"steps (Batut et al., 2013), benchmarking the use of locked nucleic acids for " -"template switching (Harbers et al., 2013), and reducing the number of primer " -"artefacts and unwanted sequences generated by ribosomal RNAs using low-" -"complexity “pseudo-random” reverse-transcription primers (Arnaud et al., " -"2016)." +"I have developed a miniaturized version of CAGE, termed **nanoCAGE**, to " +"analyse small samples yielding only nanograms of RNA (Plessy et al., 2010). " +"In the same manuscript, we also introduced its paired-end variant, " +"**CAGEscan**, which we use to **associate novel promoters with " +"annotations**. Since then, we have kept improving or expanding these " +"techniques, by updating the protocol (Salimullah et al., 2011), reducing the " +"sequence bias introduced by the molecular barcodes (Tang et al., 2013), " +"combining multiple cap-enrichment steps (Batut et al., 2013), benchmarking " +"the use of locked nucleic acids for template switching (Harbers et al., " +"2013), and reducing the number of primer artefacts and unwanted sequences " +"generated by ribosomal RNAs using low-complexity “pseudo-random” reverse-" +"transcription primers (Arnaud et al., 2016)." msgstr "" #. type: Plain text @@ -54,9 +54,9 @@ msgid "" "On April 2013, I started a new development cycle as the leader of the " "Genomics Miniaturization Technology Unit at RIKEN Center for Life Sciences, " "Division of Genomics Technology, to expand this work on single cells " -"following a population transcriptomics approach (Plessy et al., 2013) " +"following a **population transcriptomics** approach (Plessy et al., 2013) " "focused on sampling the largest possible number of cells. In our ongoing " -"developments, we have reached single-cell and single molecule resolution " +"developments, we have reached **single-cell and single molecule resolution** " "through the introduction of transposase fragmentation and unique molecular " "identifiers (Poulain et al., 2017). The protocol exists in two versions, one " "for FACS-isolated cells, and one for the Fluidigm C1 platform (Kouno et al., " @@ -74,34 +74,34 @@ msgid "" "endogenous promoter, I combined Deep-RACE, CAGE and paired-end sequencing in " "a technology that we called “Single-Locus CAGE” (Haberle et al., 2014). With " "my contributions related to CAGE development and analysis, I have been a " -"member of the FANTOM consortium since FANTOM3." +"**member of the FANTOM consortium** since FANTOM3." msgstr "" #. type: Plain text msgid "" "Together with my colleagues at RIKEN and collaborators in the field of " "neuroscience, I have applied nanoCAGE to the study of single neuron cell " -"types, for instance the olfactory neurons (Plessy et al., 2012), or in " +"types, for instance the **olfactory neurons** (Plessy et al., 2012), or in " "dopaminergic cells, where we could demonstrate the expression of haemoglobin " "in the midbrain (Biagioli et al., 2009). We are also exploring the sub-" -"cellular localisation of RNA in Purkinje neurons (Kratz et al., 2014), and " -"neurogenesis in the mouse olfactory epithelium using single-cell CAGE and " -"ATAC-seq techniques. In parallel with this promoter-centric work, I have " -"also explored the huge repertoire of the T cell antigen receptors." +"cellular localisation of RNA in **Purkinje neurons** (Kratz et al., 2014), " +"and neurogenesis in the mouse olfactory epithelium using single-cell CAGE " +"and ATAC-seq techniques. In parallel with this promoter-centric work, I have " +"also explored the huge repertoire of the **T cell antigen receptors**." msgstr "" #. type: Plain text msgid "" -"I joined OIST in 2018, to study the genetic structure and population " -"variations of an animal plankton, Oikopleura dioica, that has a genome 50 " +"I joined OIST in 2018, to study **the genetic structure and population " +"variations** of an animal plankton, Oikopleura dioica, that has a genome 50 " "time more compact than the human one, which empowers us to sequence at " "chromosomal resolution many individual sampled from all over the World." msgstr "" #. type: Plain text msgid "" -"I am also a Free Software enthusiast, and contribute to the Debian Med " -"project, by packaging bioinformatics tools, which are redistributed in " +"I am also a **Free Software** enthusiast, and contribute to the Debian Med " +"project, by **packaging bioinformatics tools**, which are redistributed in " "Debian (Möller et al., 2010) and its derivatives such as Ubuntu and " "(cloud)Bio-Linux. For digital signature of my contributions and other " "activities as a RIKEN researcher, I use the GPG key number B3443334. My " -- 2.47.3