From 43dfae8ac1908137c85ada38fbaae4c89e836177 Mon Sep 17 00:00:00 2001 From: Charles Date: Mon, 28 Jun 2021 14:58:47 +0900 Subject: [PATCH] =?utf8?q?Caf=C3=A9?= MIME-Version: 1.0 Content-Type: text/plain; charset=utf8 Content-Transfer-Encoding: 8bit --- biblio/34108687.mdwn | 20 ++++++++++++++++++++ tags/meiosis.mdwn | 8 ++++++-- 2 files changed, 26 insertions(+), 2 deletions(-) create mode 100644 biblio/34108687.mdwn diff --git a/biblio/34108687.mdwn b/biblio/34108687.mdwn new file mode 100644 index 00000000..19fbc116 --- /dev/null +++ b/biblio/34108687.mdwn @@ -0,0 +1,20 @@ +[[!meta title="Concerted cutting by Spo11 illuminates meiotic DNA break mechanics."]] +[[!tag meiosis repair]] + +Johnson D, Crawford M, Cooper T, Claeys Bouuaert C, Keeney S, Llorente B, Garcia V, Neale MJ. + +Concerted cutting by Spo11 illuminates meiotic DNA break mechanics. + +Nature. 2021 Jun;594(7864):572-576. doi:10.1038/s41586-021-03389-3 + +[[!pmid 34108687 desc="Pairs of double strand breaks created by Spo11 causes loss of short oligonucleotides, creating a cap repaired by homologous recombination."]] + +“High-resolution analysis of deproteinized Spo11-oligos showed [a ladder of] periodicity of around 10 nt [that] ranges in length from around 33 nt to more than 100 nt” “The ladder also arose in rad50S-mutant and Mre11-nuclease-defective strains of S. cerevisiae, which, like the sae2∆ mutant, cannot remove Spo11 from DSB ends. Notably, the ladder depended on the catalytic activity of Spo11” “we refer to this ladder as hyperlocalized ‘Spo11 double cuts’ (Spo11-DCs).” + +“because Spo11-DCs of less than 30 nt in length are not detected on gels and are depleted from filtered Spo11-oligo libraries, we propose that adjacent Spo11 complexes that are capable of making double cuts must, in vivo, interact with DNA from the same direction, thereby generating a ladder of Spo11-DCs with periodicity dictated by the helical pitch of DNA (around 10.5 bp).” + +“the global frequency of Spo11-DCs was disproportionately associated with regions of stronger Spo11 activity” + +“Deep sequencing of meiotic progeny identifies recombination scars that are consistent with repair initiated from gaps generated by adjacent Spo11 DSBs.” + +“We envision a mechanism in which multiple Spo11 proteins assemble with other pro-DSB factors to create a platform that enables concerted Spo11-DSB formation” diff --git a/tags/meiosis.mdwn b/tags/meiosis.mdwn index 3424eba1..be28595d 100644 --- a/tags/meiosis.mdwn +++ b/tags/meiosis.mdwn @@ -1,4 +1,8 @@ [[!meta title="pages tagged meiosis"]] -[[!inline pages="tagged(meiosis)" actions="no" archive="yes" -feedshow=10]] +_in progress_ + + - Spo11 cleaves both DNA strands. Double cleavage creates gaps repaired by + homologous recombination ([[Johnson and coll., 2021|biblio/34108687]]). + +[[!inline pages="tagged(meiosis)" limit=0]] -- 2.47.3