From 3d296e63a20f4d655c31b91736d1e97cfcb05949 Mon Sep 17 00:00:00 2001 From: Charles Date: Sun, 14 Oct 2018 07:52:37 +0900 Subject: [PATCH] bioRxiv --- biblio/10.1101_409086.mdwn | 10 ++++++++++ 1 file changed, 10 insertions(+) create mode 100644 biblio/10.1101_409086.mdwn diff --git a/biblio/10.1101_409086.mdwn b/biblio/10.1101_409086.mdwn new file mode 100644 index 00000000..c4715df7 --- /dev/null +++ b/biblio/10.1101_409086.mdwn @@ -0,0 +1,10 @@ +[[!meta title="Xdrop: targeted sequencing of long DNA molecules from low input samples using droplet sorting"]] +[[!tag emulsion Nanopore HPV]] + +Esben Bjoern Madsen, Thomas Kvist, Ida Hoijer, Adam Ameur, Marie Just Mikkelsen + +bioRxiv, September 6, 2018. + +Xdrop: targeted sequencing of long DNA molecules from low input samples using droplet sorting + +[[!doi: 10.1101/409086 desc="Enrichment by 1) encapsulating DNA fragments in double-emulsion water-in-oil-in-water droplets, 2) sorting the droplets containing the target sequence detected by a hydrolysis probe PCR, 3) extracting DNA from the droplets, 4) amplifying it by MDA (in a second generation of droplets), and finally 5) sequencing. When enriching for HPV DNA in HeLa genomes, 5 to 10% of the reads were on target. Emulsions were prepared in Dolomite chips."]] -- 2.47.3