From 38e13d28397581015596def51e9e4ebeaeac5f07 Mon Sep 17 00:00:00 2001 From: Charles Plessy Date: Fri, 29 Sep 2017 09:55:03 +0900 Subject: [PATCH] Old --- biblio/724498.mdwn | 10 ++++++++++ 1 file changed, 10 insertions(+) create mode 100644 biblio/724498.mdwn diff --git a/biblio/724498.mdwn b/biblio/724498.mdwn new file mode 100644 index 00000000..b94f7c72 --- /dev/null +++ b/biblio/724498.mdwn @@ -0,0 +1,10 @@ +[[!meta title="Reactions at the termini of tRNA with T4 RNA ligase."]] +[[!tag enzyme ligase]] + +Nucleic Acids Res. 1978 Oct;5(10):3665-77. + +Bruce AG, Uhlenbeck OC. + +Reactions at the termini of tRNA with T4 RNA ligase. + +[[!pmid 724498 desc="Optimal temperature between 0 and 15 degrees. 15-20 % DMSO enhance the reaction. pCp is easily ligated to the 3' end of tRNAs, but the 5' end of tRNAs is difficult to ligate to pCp if it is engaged in a double helix."]] -- 2.47.3