From 34eb6e3a74c2aa4051d84cb72ba81cf7f66b73a0 Mon Sep 17 00:00:00 2001 From: Charles Date: Fri, 8 Mar 2019 13:52:14 +0900 Subject: [PATCH] RNA captor --- biblio/21533245.mdwn | 10 ++++++++++ tags/cap.mdwn | 5 +++++ 2 files changed, 15 insertions(+) create mode 100644 biblio/21533245.mdwn diff --git a/biblio/21533245.mdwn b/biblio/21533245.mdwn new file mode 100644 index 00000000..dd6a5b55 --- /dev/null +++ b/biblio/21533245.mdwn @@ -0,0 +1,10 @@ +[[!meta title="RNA captor: a tool for RNA characterization."]] +[[!tag cap library method]] + +Clepet C. + +PLoS One. 2011 Apr 13;6(4):e18445. doi:10.1371/journal.pone.0018445 + +RNA captor: a tool for RNA characterization. + +[[!pmid 21533245 desc="Modified oligo-capping reaction using T4 RNA ligase 2 and a double-stranded adaptor with a 5′-protrusive NNN end and a 3′-amine blocking group on the other end of the same oligonucleotide."]] diff --git a/tags/cap.mdwn b/tags/cap.mdwn index 6e01b644..e3333e7c 100644 --- a/tags/cap.mdwn +++ b/tags/cap.mdwn @@ -37,6 +37,11 @@ Methods for enriching capped RNAs DNA ligase and a double-stranded adapter with NNNNNN overhang instead of T4 RNA ligase and a single-stranded linker. + - RNA captor ([[Clepet, 2011|biblio/21533245]]): a modified oligo-capping using + T4 RNA ligase 2 and a double-stranded adaptor. The shorter oligonucleotide in + the adaptor is blocked by an amine group on its 3′ end, and protrudes with 3 + random bases (NNN) on its 5′ end. + - [[Kwak et al., 2013|biblio/23430654]] modified oligo-capping to create Pro-cap, a method for nuclear run-on analysis at single-nucleotide resulution. -- 2.47.3