From 3002a34caca2edd2a65cc899492d584f1c988b7b Mon Sep 17 00:00:00 2001 From: Charles Plessy Date: Fri, 20 Oct 2017 17:36:15 +0900 Subject: [PATCH] Old --- biblio/4000951.mdwn | 10 ++++++++++ biblio/To_Do | 3 --- 2 files changed, 10 insertions(+), 3 deletions(-) create mode 100644 biblio/4000951.mdwn diff --git a/biblio/4000951.mdwn b/biblio/4000951.mdwn new file mode 100644 index 00000000..7ea38871 --- /dev/null +++ b/biblio/4000951.mdwn @@ -0,0 +1,10 @@ +[[!meta title="Hexamine cobalt chloride promotes intermolecular ligation of blunt end DNA fragments by T4 DNA ligase."]] +[[!tag ligation method]] + +Nucleic Acids Res. 1985 Mar 25;13(6):1997-2008. + +Rusche JR, Howard-Flanders P. + +Hexamine cobalt chloride promotes intermolecular ligation of blunt end DNA fragments by T4 DNA ligase. + +[[!pmid 4000951 desc="HCC (1 mM) promotes intermolecular ligation. This effect is countered by monovalent cations."]] diff --git a/biblio/To_Do b/biblio/To_Do index dffec3ea..fa189bd6 100644 --- a/biblio/To_Do +++ b/biblio/To_Do @@ -667,9 +667,6 @@ More inert than glycogen. 12448877 [protocols] Ligating a DNA adaptor 3' to the FScDNA using T4RNA ligase. -4000951 [protocols] -HCC (1 mM) promotes intermolecular ligation. This effect is countered by monovalent cations. - 386284 [protocols] A low ATP concentration contributes to the attainment of high yields. ATP regenerating systems allow to work at sub-stoechiometric concentrations. Rnase A can be used as a SSBP. Spermine enhances yield. -- 2.47.3