From 21e91fb8907f36c3da3197e4c917d2f0e053e1ad Mon Sep 17 00:00:00 2001 From: Charles Plessy Date: Wed, 1 Nov 2017 13:13:05 +0900 Subject: [PATCH] Minor edits. --- tags/cap.mdwn | 16 +++++++++------- 1 file changed, 9 insertions(+), 7 deletions(-) diff --git a/tags/cap.mdwn b/tags/cap.mdwn index 27bfd583..78ca0f35 100644 --- a/tags/cap.mdwn +++ b/tags/cap.mdwn @@ -6,16 +6,18 @@ Methods for enriching capped RNAs. - A method similar to oligo-capping was reported by [[Sekine and Kato|biblio/8247743]] in 1993. - - Oligo-capping [[Suzuki et al., 1999|biblio/9373149]]: dephosphorylate, uncover functional - phosphate with decapping enzyme, and ligate RNA adaptor with T4 RNA ligase. + - Oligo-capping ([[Suzuki et al., 1999|biblio/9373149]]): dephosphorylate, + uncover functional phosphate with decapping enzyme, and ligate RNA adaptor + with T4 RNA ligase. (wip: Fromont-racine et al., Maruyama et al.) - - CapSelect [[Schmidt et al., 1999|biblio/10518626]]: Add a poly-A tail to the first-strand - cDNA, ligate a double-stranded adapter ending with a single-strand (T)TTTGGG - overhang, and prime second-strand synthesis. + - CapSelect ([[Schmidt et al., 1999|biblio/10518626]]): Add a poly-A tail to + the first-strand cDNA, ligate a double-stranded adapter ending with a + single-strand (T)TTTGGG overhang, and prime second-strand synthesis. - - [[Cleptet et al., 2004|biblio/14704363]] used oligo-capping, but used T4 DNA ligase and a double-stranded - adapter with NNNNNN overhang instead of T4 RNA ligase and a single-stranded linker. + - [[Cleptet et al., 2004|biblio/14704363]] modified oligo-capping, to use T4 + DNA ligase and a double-stranded adapter with NNNNNN overhang instead of T4 + RNA ligase and a single-stranded linker. [[!inline pages="tagged(cap)" limit=0]] -- 2.47.3