From 1619e61c07bd712c09bce3cd765c9ebc795710a9 Mon Sep 17 00:00:00 2001 From: Charles Date: Wed, 2 Sep 2020 15:05:00 +0900 Subject: [PATCH] =?utf8?q?Caf=C3=A9?= MIME-Version: 1.0 Content-Type: text/plain; charset=utf8 Content-Transfer-Encoding: 8bit --- biblio/1465136.mdwn | 10 ++++++++++ tags/trans-splicing.mdwn | 3 +++ 2 files changed, 13 insertions(+) create mode 100644 biblio/1465136.mdwn diff --git a/biblio/1465136.mdwn b/biblio/1465136.mdwn new file mode 100644 index 00000000..1039f0b1 --- /dev/null +++ b/biblio/1465136.mdwn @@ -0,0 +1,10 @@ +[[!meta title="Spliced leader RNAs from lower eukaryotes are trans-spliced in mammalian cells."]] +[[!tag trans-splicing]] + +Bruzik JP, Maniatis T. + +Nature. 1992 Dec 17;360(6405):692-5. doi:10.1038/360692a0 + +Spliced leader RNAs from lower eukaryotes are trans-spliced in mammalian cells. + +[[!pmid 1465136 desc="Trans-splicing in vivo in COS cells and in vitro in HeLa cell extracts, using splice leaders and actin genes from C. elegans and Leptomonas colosoma, and also using an adenoviral RNA as acceptor."]] diff --git a/tags/trans-splicing.mdwn b/tags/trans-splicing.mdwn index 7ded5bdd..0a750e29 100644 --- a/tags/trans-splicing.mdwn +++ b/tags/trans-splicing.mdwn @@ -1,5 +1,8 @@ [[!meta title="pages tagged trans-splicing"]] +Trans-spplicing of a splice leader from a nematode and a trypanosome was shown +to be possible in COS cells and HeLa cell extracts by [[Bruzik and Maniatis, 1992|biblio/1465136]]. + Trans-splicing was discovered in tunicates by [[Vandenberghe, Meedel and Hastings, 2001|biblio/11159910]]. The splice leader is 16-nt in C. intestinalis ([[Satou et al, 2006|biblio/16822859]]) and 40-nt in O. dioica ([[Ganot et al., 2004|biblio/15314184]]). -- 2.47.3