From 14da08380b29a046e91ff96585e2708108d299c3 Mon Sep 17 00:00:00 2001
From: Charles Plessy <https://launchpad.net/~plessy>
Date: Wed, 4 Jun 2014 18:15:05 +0900
Subject: [PATCH] =?utf8?q?C'=C3=A9tait=20l'heure=20de=20la=20soupe.?=
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+[[!meta title="Sensitivity and accuracy of quantitative real-time polymerase chain reaction using SYBR green I depends on cDNA synthesis conditions."]]
+[[!tag DTT reverse_transcription qPCR]]
+
+Lekanne Deprez RH, Fijnvandraat AC, Ruijter JM, Moorman AF.
+
+Anal Biochem. 2002 Aug 1;307(1):63-9.
+
+Sensitivity and accuracy of quantitative real-time polymerase chain reaction using SYBR green I depends on cDNA synthesis conditions.
+
+[[!pmid 12137780 desc="DTT interferes with fluorescence measurements in qPCR.  RT without DTT is efficient.  Random-primerd replicates are less reproducible than oligo-dT or gene-specific-primed ones."]]
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2.47.3