From 12cd74d3921bd0eaf8a9592cd656d1ff90f6845a Mon Sep 17 00:00:00 2001 From: Charles Plessy Date: Tue, 15 May 2018 09:31:06 +0900 Subject: [PATCH] slack --- biblio/26152304.mdwn | 10 ++++++++++ 1 file changed, 10 insertions(+) create mode 100644 biblio/26152304.mdwn diff --git a/biblio/26152304.mdwn b/biblio/26152304.mdwn new file mode 100644 index 00000000..41a1fb83 --- /dev/null +++ b/biblio/26152304.mdwn @@ -0,0 +1,10 @@ +[[!meta title="A new method to prevent carry-over contaminations in two-step PCR NGS library preparations."]] +[[!tag library amplification method]] + +Seitz V, Schaper S, Dröge A, Lenze D, Hummel M, Hennig S. + +Nucleic Acids Res. 2015 Nov 16;43(20):e135. doi:10.1093/nar/gkv694 + +A new method to prevent carry-over contaminations in two-step PCR NGS library preparations. + +[[!pmid 26152304 desc="Proposes to introduce indexes during the cDNA PCR, and to partially match them during Library PCR, so that 1) the PCR is more stringent and 2) contaminations can be detected by sequence analysis."]] -- 2.47.3