From 0ff9009e8e87aaec540cb5ad83f5b30203f20c51 Mon Sep 17 00:00:00 2001 From: Charles Date: Mon, 20 Jan 2020 00:59:12 +0900 Subject: [PATCH] =?utf8?q?Caf=C3=A9?= MIME-Version: 1.0 Content-Type: text/plain; charset=utf8 Content-Transfer-Encoding: 8bit --- biblio/31740957.mdwn | 10 ++++++++++ 1 file changed, 10 insertions(+) create mode 100644 biblio/31740957.mdwn diff --git a/biblio/31740957.mdwn b/biblio/31740957.mdwn new file mode 100644 index 00000000..53198ec2 --- /dev/null +++ b/biblio/31740957.mdwn @@ -0,0 +1,10 @@ +[[!meta title="DNA barcoding reveals that injected transgenes are predominantly processed by homologous recombination in mouse zygote."]] +[[!tag recombination repair]] + +Smirnov A, Fishman V, Yunusova A, Korablev A, Serova I, Skryabin BV, Rozhdestvensky TS, Battulin N. + +Nucleic Acids Res. 2020 Jan 24;48(2):719-735. doi:10.1093/nar/gkz1085 + +DNA barcoding reveals that injected transgenes are predominantly processed by homologous recombination in mouse zygote. + +[[!pmid 31740957 desc="A library of constructs bearing a different barcode at each end was injected in mouse pronuclei and the resulting transgenes were sequenced from 10 embryos. New combinations of barcodes were observed in the majority of the copies inside concatemers. The data does not support the hypothesis of a rolling circle replication of individual constructs. The model is: first, some linear constructs are concatemerised by NHEJ (non-homologous end joining), then HR (homologous recombination) incorporates extra copies, causing exchange of barcodes as side effect."]] -- 2.47.3