From 0edcc390dd4432c92c13e0f97e1540cf398daeac Mon Sep 17 00:00:00 2001 From: Charles Plessy Date: Thu, 31 Aug 2017 09:23:39 +0900 Subject: [PATCH] vieux. --- biblio/15786810.mdwn | 10 ++++++++++ biblio/To_Do | 3 --- 2 files changed, 10 insertions(+), 3 deletions(-) create mode 100644 biblio/15786810.mdwn diff --git a/biblio/15786810.mdwn b/biblio/15786810.mdwn new file mode 100644 index 00000000..15b4888e --- /dev/null +++ b/biblio/15786810.mdwn @@ -0,0 +1,10 @@ +[[!meta title="cDNA library construction from a small amount of RNA: adaptor-ligation approach for two-round cRNA amplification using T7 and SP6 RNA polymerases."]] +[[!tag ]] + +Biotechniques. 2005 Mar;38(3):451-8. + +Ohara R, Kikuno RF, Kitamura H, Ohara O. + +cDNA library construction from a small amount of RNA: adaptor-ligation approach for two-round cRNA amplification using T7 and SP6 RNA polymerases. + +[[!pmid 15786810 desc="High size is preserved by ligating a 5'adapter before each amplification."]] diff --git a/biblio/To_Do b/biblio/To_Do index 1654f0b8..9d4e9ba3 100644 --- a/biblio/To_Do +++ b/biblio/To_Do @@ -449,9 +449,6 @@ Template matching approach on expression vectors. 15785770 [misc] Future Nobel prize ? Maybe an explanation for the conservation of dev. enhancers. -15786810 [libraries] -High size is preserved by ligating a 5'adapter before each amplification. - 15778292 [genome] 2n species covered 1x would be more efficient than n covered 2x for the discovery of conserved elements, but the resulting genome assemblies would be of a much poorer qualities. -- 2.47.3