From 0c23b6ab292f043507ac0b715893bf07b1341105 Mon Sep 17 00:00:00 2001 From: Charles Plessy Date: Mon, 13 May 2019 12:01:05 +0900 Subject: [PATCH] MthRnl --- biblio/18829718.mdwn | 10 ++++++++++ tags/ligase.mdwn | 9 ++++++++- 2 files changed, 18 insertions(+), 1 deletion(-) create mode 100644 biblio/18829718.mdwn diff --git a/biblio/18829718.mdwn b/biblio/18829718.mdwn new file mode 100644 index 00000000..13248de7 --- /dev/null +++ b/biblio/18829718.mdwn @@ -0,0 +1,10 @@ +[[!meta title="Archaeal RNA ligase is a homodimeric protein that catalyzes intramolecular ligation of single-stranded RNA and DNA."]] +[[!tag enzyme ligase adenylylation]] + +Torchia C, Takagi Y, Ho CK. + +Nucleic Acids Res. 2008 Nov;36(19):6218-27. doi:10.1093/nar/gkn602 + +Archaeal RNA ligase is a homodimeric protein that catalyzes intramolecular ligation of single-stranded RNA and DNA. + +[[!pmid 18829718 desc="Used in the NEB's 5´ DNA Adenylation Kit. Lacks specificity for RNA and DNA."]] diff --git a/tags/ligase.mdwn b/tags/ligase.mdwn index baa0d3c7..2ba035e9 100644 --- a/tags/ligase.mdwn +++ b/tags/ligase.mdwn @@ -13,7 +13,7 @@ On T4 RNL1: Adenylation efficiency after 6h: C >> U ~ A > G ([[McLaughlin et al., 1985|biblio/3978074]]). - Also used for single-strand DNA ligation ([[Tessier, Brousserau & Vernet, 1986|biblio/3799962]]) with PEG and hexamine cobalt chloride (HCC) as additives. - - Can dephoshporylate RNA 3′ ends ([[Krug & Uhlenbeck, 1982|biblio/7082652]]). + - Can dephoshporylate RNA 3′ ends ([[Krug & Uhlenbeck, 1982|biblio/7082652]]). On T4 RNL2: @@ -37,12 +37,19 @@ DNA and RNA ligases can be used to adenylylate a substrate. - [[Hoffmann and McLaughlin (1987)|biblio/3299268]] used T4 RNL 1 to adenylylate RNA, and noted that ssNpR(A) are good acceptors, and pCpN are good donors. + - [[Wang and Unrau (2002)|biblio/12503310]] noted that purified + recombinant ligase can be partly adenylylated, which blocks the + reaction on pre-adenylylated substrates. Pre-incubation with + pyrophosphate can displace AMP moiety. Commercial ligases + are reported to contain mostly non-adenylylated proteins. - [[Ho, Wang, Lima and Shuman (2004)|biblio/14962393]] reported the use of RNL(1-249) on pre-adenylylated RNA in absence of ATP for avoiding concatemerisation. - [[Silverman (2004)|biblio/15037782]] reported the use a complementary DNA oligonucleotide (leaving 3-4 protruding 5'ribonucleotides) when adenylylating with T4 RNL 1. + - [[Torchia, Takagi and Ho (2008)|biblio/18829718]] reported the use + of the Methanobacterium RNA ligase (MthRnl) to adenylylate RNA. [[!inline pages="tagged(ligase)" limit=0]] [[!meta title="pages tagged adenylylation"]] -- 2.47.3