From 09d4b081ce9f6af83d81281091020f37a01543ad Mon Sep 17 00:00:00 2001 From: Charles Plessy Date: Thu, 19 Oct 2017 10:29:03 +0900 Subject: [PATCH] ActD. --- tags/reverse_transcription.mdwn | 12 ++++++++++-- 1 file changed, 10 insertions(+), 2 deletions(-) diff --git a/tags/reverse_transcription.mdwn b/tags/reverse_transcription.mdwn index 7d2e930a..04109217 100644 --- a/tags/reverse_transcription.mdwn +++ b/tags/reverse_transcription.mdwn @@ -1,4 +1,12 @@ [[!meta title="pages tagged reverse transcription"]] -[[!inline pages="tagged(reverse_transcription)" actions="no" archive="yes" -feedshow=10]] +(redaction in progress) + +Reverse-transcriptases have a DNA-dependent DNA polymerase activity. + - It is utilised in [[template_switching]] methods to add linkers to first-strand cDNAs. + - It is also a source of antisense artefacts when the RT makes a second-strand cDNA + that is mistaken for a first-strand cDNA. ActinomycinD inhibits DNA-dependent, + but not RNA-dependent polymerase activity and is used to suppress these + artefacts ([[Perocchi et al., 2007|biblio/17897965]], [[Kanamori-Katayama et al., 2011|biblio/21596820]]). + +[[!inline pages="tagged(reverse_transcription)" actions="no" limit=0]] -- 2.47.3