From: Charles Plessy <plessy@riken.jp>
Date: Wed, 16 May 2018 07:23:07 +0000 (+0900)
Subject: NMP
X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=ff7bffb4854a8e664ebfea10ccd2035466ee2dfc;p=source%2F.git

NMP
---

diff --git a/biblio/28150748.mdwn b/biblio/28150748.mdwn
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+[[!meta title="Efficient N-tailing of blunt DNA ends by Moloney murine leukemia virus reverse transcriptase."]]
+[[!tag reverse-transcription]]
+
+Sci Rep. 2017 Feb 2;7:41769. doi:10.1038/srep41769
+
+Ohtsubo Y, Nagata Y, Tsuda M.
+
+Efficient N-tailing of blunt DNA ends by Moloney murine leukemia virus reverse transcriptase.
+
+[[!pmid 28150748 desc="Specific tailing of first-strand cDNAs is robustly enhanced by the complementary dNMP (C enhanced by dGMP, etc.).  A-tailing is not enhanced, perhaps because it is already very strong.  Reactions made in presence of manganese; not tested with only magnesium."]]
diff --git a/biblio/3323527.mdwn b/biblio/3323527.mdwn
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+[[!meta title="Novel blunt-end addition reactions catalyzed by DNA polymerase I of Escherichia coli."]]
+[[!tag enzyme]]
+
+J Mol Biol. 1987 Nov 5;198(1):123-7.
+
+Clark JM, Joyce CM, Beardsley GP.
+
+Novel blunt-end addition reactions catalyzed by DNA polymerase I of Escherichia coli.
+
+[[!pmid 3323527 desc="Reports enhancement of +1 tailing by Klenow fragment with dNMPs."]]
diff --git a/biblio/7507249.mdwn b/biblio/7507249.mdwn
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+[[!meta title="Marked infidelity of human immunodeficiency virus type 1 reverse transcriptase at RNA and DNA template ends."]]
+[[!tag reverse_transcription]]
+
+Proc Natl Acad Sci U S A. 1994 Jan 18;91(2):549-53 doi:10.1073/pnas.91.2.549
+
+Patel PH & Preston BD.
+
+Marked infidelity of human immunodeficiency virus type 1 reverse transcriptase at RNA and DNA template ends.
+
+[[!pmid 7507249 desc="On DNA/DNA blunt ends, adds a single nucleotide (A > G >> C|T).  On RNA/DNA blund ends, adds more nucleotides.  Addition is favoured by increased dNTP levels."]]
diff --git a/tags/reverse_transcription.mdwn b/tags/reverse_transcription.mdwn
index 696155c1..6866b55e 100644
--- a/tags/reverse_transcription.mdwn
+++ b/tags/reverse_transcription.mdwn
@@ -24,12 +24,22 @@ _(redaction in progress)_
 Like other DNA polymerases ([[Clark, 1988|biblio/2460825]]), reverse
 transcriptases have a TdT activity.
 
+ - [[Patel & Preston, 1994|biblio/7507249]] showed that HIV RT adds one
+   nucleotide (A > G >> T|C) on DNA/DNA duplexes, and more on DNA/RNA duplexes.
+   Addition is favoured by increased dNTP levels.  MMLV and AMV were also
+   reported (data not shown) to add multiple nucleotides.
+
  - [[Chen & Patton, 2001|biblio/11252793]] reported a TdT activity for MMLV and
    AMV, with a preference for adding As.  For MMLV, activity reduced abruptly
    between 45 and 50 °C.  For AMV, it decreased constantly from 25 to 50 °C.
    Activity increased with concentration for MMLV.  (High-concentration AMV was
    not available.)
 
+ - Following an initial observation of [[Clark et al (1987)|biblio/3323527]] on
+   the Klenow fragment, [[Ohtsubo et al, 2017|biblio/28150748]] showed that
+   specific tailing is enhanced by the complementary dNMP (C enhanced by dGMP,
+   etc.), except for A-tailing, which is already the strongest.
+
 ### Reverse-transcriptases tolerate terminal mismatches
 
  - Reported by [[Mizuno et al., 1999|biblio/9973624]].